Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-LIPE Recombinant Antibody (CBYJL-1772) (CBMAB-L1684-YJ)


See all LIPE antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1772
Antibody Isotype
IgG2b
Application
IHC, WB

Basic Information

Immunogen
E. coli-derived recombinant human Hormone-sensitive Lipase/HSL Met302-Leu425 Accession # Q05469.ImmunogenType: Recombinant protein.
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
lipase E, hormone sensitive type
Introduction
LIPE has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
Entrez Gene ID
3991
UniProt ID
Q05469
Alternative Names
HSL; LHS; AOMS4; FPLD6
Function
Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:8812477, PubMed:15955102, PubMed:15716583, PubMed:19800417).
Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed:19800417).
Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity).
Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity).
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).
Biological Process
Cholesterol metabolic processIEA:UniProtKB-KW
Diacylglycerol catabolic processISS:UniProtKB
Ether lipid metabolic processISS:UniProtKB
Lipid catabolic processISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Triglyceride catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell membrane
Membrane, caveola
Cytoplasm, cytosol
Lipid droplet
Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959).
Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity).
Involvement in disease
Lipodystrophy, familial partial, 6 (FPLD6):
A form of lipodystrophy characterized by abnormal subcutaneous fat distribution. Affected individuals have increased visceral fat, impaired lipolysis, dyslipidemia, hepatic steatosis, systemic insulin resistance, and diabetes. Some patients manifest muscular dystrophy.
PTM
Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-LIPE Recombinant Antibody (CBYJL-1772)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry