Mouse Anti-LIG3 Recombinant Antibody (4H9) (CBMAB-L1531-YJ)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4H9

Antibody Isotype

IgG1

Application

IP, WB

Basic Information

Immunogen

Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-919

Target

Full Name

LIG3

Introduction

LIG3 is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.Diseases associated with LIG3 include Cloacal Exstrophy and Bloom Syndrome. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Transcription-Coupled Nucleotide Excision Repair (TC-NER). Gene Ontology (GO) annotations related to this gene include DNA ligase activity and DNA ligase (ATP) activity. An important paralog of this gene is LIG1.Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.

Entrez Gene ID

3980

UniProt ID

P49916

Alternative Names

LIG2

Function

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).

Biological Process

Base-excision repair, DNA ligationManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIDA:BHF-UCL
Double-strand break repairManual Assertion Based On ExperimentIDA:BHF-UCL
Double-strand break repair via alternative nonhomologous end joiningManual Assertion Based On ExperimentIGI:BHF-UCL
Double-strand break repair via homologous recombinationTAS:Reactome
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mitochondrial DNA repairManual Assertion Based On ExperimentIMP:BHF-UCL
Mitochondrion organizationIDA:BHF-UCL
Negative regulation of mitochondrial DNA replicationManual Assertion Based On ExperimentIMP:CACAO

Cellular Location

Isoform 1&2:
Mitochondrion
Contains an N-terminal mitochondrial transit peptide.
Isoform 3&4:
Nucleus
Lacks the N-terminal mitochondrial transit peptide.

More Infomation

References

McNally, J. R., Ames, A. M., Admiraal, S. J., & O’Brien, P. J. (2023). Human DNA ligases I and III have stand-alone end-joining capability, but differ in ligation efficiency and specificity. Nucleic Acids Research, 51(2), 796-805.

Dias, M. P., Tripathi, V., van der Heijden, I., Cong, K., Manolika, E. M., Bhin, J., ... & Jonkers, J. (2021). Loss of nuclear DNA ligase III reverts PARP inhibitor resistance in BRCA1/53BP1 double-deficient cells by exposing ssDNA gaps. Molecular cell, 81(22), 4692-4708.

Caracciolo, D., Di Martino, M. T., Amodio, N., Morelli, E., Montesano, M., Botta, C., ... & Tassone, P. (2019). miR-22 suppresses DNA ligase III addiction in multiple myeloma. Leukemia, 33(2), 487-498.

McNally, J. (2019). Kinetic Analysis of Human DNA Ligase III (Doctoral dissertation).

Hu, Y., Lin, J., Fang, H., Fang, J., Li, C., Chen, W., ... & Zhao, J. J. (2018). Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and apoptosis in multiple myeloma. Leukemia, 32(10), 2250-2262.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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