Mouse Anti-LIG3 Recombinant Antibody (1F3) (CBMAB-D2108-YC)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Chicken, Mouse

Clone

1F3

Antibody Isotype

IgG1

Application

ICC, IF, Neut, WB

Basic Information

Immunogen

Recombinant full-length human DNA Ligase III-b

Specificity

Human, Chicken, Mouse

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

LIG3

Introduction

LIG3 is a member of the DNA ligase family. LIG3 is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus.

Entrez Gene ID

Human	3980
Mouse	16882
Chicken	417530

UniProt ID

Human	P49916
Mouse	P97386
Chicken	F1P394

Alternative Names

LIG2

Function

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).

Biological Process

Base-excision repair, DNA ligationManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIDA:BHF-UCL
Double-strand break repairManual Assertion Based On ExperimentIDA:BHF-UCL
Double-strand break repair via alternative nonhomologous end joiningManual Assertion Based On ExperimentIGI:BHF-UCL
Double-strand break repair via homologous recombinationTAS:Reactome
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mitochondrial DNA repairManual Assertion Based On ExperimentIMP:BHF-UCL
Mitochondrion organizationIDA:BHF-UCL
Negative regulation of mitochondrial DNA replicationManual Assertion Based On ExperimentIMP:CACAO

Cellular Location

Isoform 1&2:
Mitochondrion
Contains an N-terminal mitochondrial transit peptide.
Isoform 3&4:
Nucleus
Lacks the N-terminal mitochondrial transit peptide.

More Infomation

References

McNally, J. R., Ames, A. M., Admiraal, S. J., & O’Brien, P. J. (2023). Human DNA ligases I and III have stand-alone end-joining capability, but differ in ligation efficiency and specificity. Nucleic Acids Research, 51(2), 796-805.

Dias, M. P., Tripathi, V., van der Heijden, I., Cong, K., Manolika, E. M., Bhin, J., ... & Jonkers, J. (2021). Loss of nuclear DNA ligase III reverts PARP inhibitor resistance in BRCA1/53BP1 double-deficient cells by exposing ssDNA gaps. Molecular cell, 81(22), 4692-4708.

Caracciolo, D., Di Martino, M. T., Amodio, N., Morelli, E., Montesano, M., Botta, C., ... & Tassone, P. (2019). miR-22 suppresses DNA ligase III addiction in multiple myeloma. Leukemia, 33(2), 487-498.

McNally, J. (2019). Kinetic Analysis of Human DNA Ligase III (Doctoral dissertation).

Hu, Y., Lin, J., Fang, H., Fang, J., Li, C., Chen, W., ... & Zhao, J. J. (2018). Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and apoptosis in multiple myeloma. Leukemia, 32(10), 2250-2262.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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Second antibody and isotype control

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