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Mouse Anti-LIG1 Recombinant Antibody (CBYJL-1659) (CBMAB-L1528-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1659
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
LIG1 (NP_000225, 810 a.a. ~ 919 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: QSLKALVLPS PRPYVRIDGA VIPDHWLDPS AVWEVKCADL SLSPIYPAAR GLVDSDKGIS LRFPRFIRVR EDKQPEQATT SAQVACLYRK QSQIQNQQGE DSGSDPEDTY.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 810-919

Target

Full Name
DNA Ligase 1
Introduction
LIG1 is a member of the ATP-dependent DNA ligase protein family. LIG1 functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. Diseases associated with LIG1 include Bloom Syndrome and Achalasia-Addisonianism-Alacrima Syndrome. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Cell Cycle, Mitotic. Gene Ontology (GO) annotations related to this gene include DNA ligase activity and DNA ligase (ATP) activity. An important paralog of this gene is LIG3.DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Entrez Gene ID
3978
UniProt ID
P18858
Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Base-excision repairManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIBA:GO_Central
DNA recombinationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mismatch repairTAS:Reactome
Okazaki fragment processing involved in mitotic DNA replicationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
More Infomation

Sallmyr, A., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2023). Mammalian DNA ligases; roles in maintaining genome integrity. Journal of Molecular Biology, 168276.

Silva, J. M., Jones, A., Sibson, K., Bibi, S., Jeggo, P., Woodbine, L., ... & Amrolia, P. J. (2021). Haematopoietic stem cell transplantation for DNA ligase 1 deficiency. Journal of Clinical Immunology, 41, 238-242.

Jurkiw, T. J., Tumbale, P. P., Schellenberg, M. J., Cunningham-Rundles, C., Williams, R. S., & O’Brien, P. J. (2021). LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency. Nucleic acids research, 49(3), 1619-1630.

Sallmyr, A., Rashid, I., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2020). Human DNA ligases in replication and repair. DNA repair, 93, 102908.

Tang, Q., Kamble, P., & Çağlayan, M. (2020). DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage. Mutagenesis, 35(5), 391-404.

Tomkinson, A. E., Naila, T., & Khattri Bhandari, S. (2020). Altered DNA ligase activity in human disease. Mutagenesis, 35(1), 51-60.

Liddiard, K., Ruis, B., Kan, Y., Cleal, K., Ashelford, K. E., Hendrickson, E. A., & Baird, D. M. (2019). DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase. Nucleic acids research, 47(5), 2402-2424.

Maffucci, P., Chavez, J., Jurkiw, T. J., O’Brien, P. J., Abbott, J. K., Reynolds, P. R., ... & Cunningham-Rundles, C. (2018). Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. The Journal of clinical investigation, 128(12), 5489-5504.

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For research use only. Not intended for any clinical use.

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