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Mouse Anti-LIG1 Recombinant Antibody (7A12) (CBMAB-D2101-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Cattle
Clone
7A12
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Bovine DNA Ligase I
Specificity
Human, Cattle
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Ligase 1
Introduction
LIG1 is a member of the ATP-dependent DNA ligase protein family. LIG1 functions in DNA replication, recombination, and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of LIG1 may also be associated with a variety of cancers.
Entrez Gene ID
Human3978
Cattle100124507
UniProt ID
HumanQ96JA1
CattleA0A3S5ZPU0
Alternative Names
DNA Ligase 1
Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Base-excision repairManual Assertion Based On ExperimentIDA:BHF-UCL
Base-excision repair, gap-fillingTAS:Reactome
Cell divisionIEA:UniProtKB-KW
DNA biosynthetic processIEA:InterPro
DNA ligationManual Assertion Based On ExperimentIBA:GO_Central
DNA recombinationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Lagging strand elongationManual Assertion Based On ExperimentIBA:GO_Central
Mismatch repairTAS:Reactome
Okazaki fragment processing involved in mitotic DNA replicationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
More Infomation

Sallmyr, A., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2023). Mammalian DNA ligases; roles in maintaining genome integrity. Journal of Molecular Biology, 168276.

Silva, J. M., Jones, A., Sibson, K., Bibi, S., Jeggo, P., Woodbine, L., ... & Amrolia, P. J. (2021). Haematopoietic stem cell transplantation for DNA ligase 1 deficiency. Journal of Clinical Immunology, 41, 238-242.

Jurkiw, T. J., Tumbale, P. P., Schellenberg, M. J., Cunningham-Rundles, C., Williams, R. S., & O’Brien, P. J. (2021). LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency. Nucleic acids research, 49(3), 1619-1630.

Sallmyr, A., Rashid, I., Bhandari, S. K., Naila, T., & Tomkinson, A. E. (2020). Human DNA ligases in replication and repair. DNA repair, 93, 102908.

Tang, Q., Kamble, P., & Çağlayan, M. (2020). DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage. Mutagenesis, 35(5), 391-404.

Tomkinson, A. E., Naila, T., & Khattri Bhandari, S. (2020). Altered DNA ligase activity in human disease. Mutagenesis, 35(1), 51-60.

Liddiard, K., Ruis, B., Kan, Y., Cleal, K., Ashelford, K. E., Hendrickson, E. A., & Baird, D. M. (2019). DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase. Nucleic acids research, 47(5), 2402-2424.

Maffucci, P., Chavez, J., Jurkiw, T. J., O’Brien, P. J., Abbott, J. K., Reynolds, P. R., ... & Cunningham-Rundles, C. (2018). Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. The Journal of clinical investigation, 128(12), 5489-5504.

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For research use only. Not intended for any clinical use.

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