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Mouse Anti-LGALS3 Recombinant Antibody (CBYCL-268) (CBMAB-L0169-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYCL-268
Antibody Isotype
IgG1, κ
Application
IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4, with 0.09% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 163-191

Target

Full Name
galectin 3
Introduction
LGALS3 is a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi.
Entrez Gene ID
3958
UniProt ID
P17931
Alternative Names
L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Biological Process
Antimicrobial humoral immune response mediated by antimicrobial peptideIDA:UniProtKB
Eosinophil chemotaxisManual Assertion Based On ExperimentIDA:BHF-UCL
Epithelial cell differentiationManual Assertion Based On ExperimentIEP:UniProtKB
Innate immune responseIEA:UniProtKB-KW
Killing of cells of other organismIDA:UniProtKB
Macrophage chemotaxisManual Assertion Based On ExperimentIDA:BHF-UCL
Monocyte chemotaxisManual Assertion Based On ExperimentIDA:BHF-UCL
Mononuclear cell migrationManual Assertion Based On ExperimentIDA:BHF-UCL
mRNA processingIEA:UniProtKB-KW
Negative regulation of endocytosisManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of immunological synapse formationBy SimilarityISS:BHF-UCL
Negative regulation of protein tyrosine phosphatase activity1 PublicationIC:ARUK-UCL
Negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cellBy SimilarityISS:BHF-UCL
Negative regulation of T cell receptor signaling pathwayBy SimilarityISS:BHF-UCL
Neutrophil chemotaxisManual Assertion Based On ExperimentIDA:BHF-UCL
Positive chemotaxisManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of calcium ion importManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of mononuclear cell migrationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of protein localization to plasma membraneManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of protein-containing complex assemblyManual Assertion Based On ExperimentIDA:ARUK-UCL
Regulation of extrinsic apoptotic signaling pathway via death domain receptorsManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of T cell apoptotic processManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of T cell proliferationManual Assertion Based On ExperimentIMP:BHF-UCL
RNA splicingIEA:UniProtKB-KW
Cellular Location
Cytoplasm
Nucleus
Secreted
Secreted by a non-classical secretory pathway and associates with the cell surface. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059).
More Infomation

Burbidge, K., Rademacher, D. J., Mattick, J., Zack, S., Grillini, A., Bousset, L., ... & Campbell, E. M. (2022). LGALS3 (galectin 3) mediates an unconventional secretion of SNCA/α-synuclein in response to lysosomal membrane damage by the autophagic-lysosomal pathway in human midbrain dopamine neurons. Autophagy, 18(5), 1020-1048.

Xu, W. D., Wu, Q., He, Y. W., Huang, A. F., Lan, Y. Y., Fu, L., ... & Liu, X. Y. (2021). Gene polymorphisms of LGALS2, LGALS3 and LGALS9 in patients with rheumatoid arthritis. Cellular Immunology, 368, 104419.

Rong, J., Pan, H., He, J., Zhang, Y., Hu, Y., Wang, C., ... & Zheng, Z. (2020). Long non-coding RNA KCNQ1OT1/microRNA-204-5p/LGALS3 axis regulates myocardial ischemia/reperfusion injury in mice. Cellular Signalling, 66, 109441.

Hu, W. M., Yang, Y. Z., Zhang, T. Z., Qin, C. F., & Li, X. N. (2020). LGALS3 is a poor prognostic factor in diffusely infiltrating gliomas and is closely correlated with CD163+ tumor-associated macrophages. Frontiers in Medicine, 7, 182.

Takashima, Y., Kawaguchi, A., Hayano, A., & Yamanaka, R. (2019). CD276 and the gene signature composed of GATA3 and LGALS3 enable prognosis prediction of glioblastoma multiforme. PloS one, 14(5), e0216825.

Li, Z., Lv, F., Dai, C., Wang, Q., Jiang, C., Fang, M., & Xu, Y. (2019). Activation of galectin-3 (LGALS3) transcription by injurious stimuli in the liver is commonly mediated by BRG1. Frontiers in cell and developmental biology, 7, 310.

Wang, H., Song, X., Huang, Q., Xu, T., Yun, D., Wang, Y., ... & Chen, J. (2019). LGALS3 promotes treatment resistance in glioblastoma and is associated with tumor risk and prognosis. Cancer Epidemiology, Biomarkers & Prevention, 28(4), 760-769.

He, X., Zhang, S., Chen, J., & Li, D. (2019). Increased LGALS3 expression independently predicts shorter overall survival in patients with the proneural subtype of glioblastoma. Cancer Medicine, 8(5), 2031-2040.

Zhang, Y., Wang, Y., Zhai, M., Gan, T., Zhao, X., Zhang, R., ... & Zhang, J. (2018). Influence of LGALS3 gene polymorphisms on susceptibility and prognosis of dilated cardiomyopathy in a Northern Han Chinese population. Gene, 642, 293-298.

Chaudoin, T. R., & Bonasera, S. J. (2018). Mice lacking galectin-3 (Lgals3) function have decreased home cage movement. BMC neuroscience, 19(1), 1-10.

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For research use only. Not intended for any clinical use.

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