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Mouse Anti-LAMC1 Recombinant Antibody (BC17) (CBMAB-L0612-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
BC17
Antibody Isotype
IgG1
Application
IHC, IP

Basic Information

Immunogen
Monoclonal antibody (Mab) to laminin g1-chain is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Pepsin-digested laminins from human placenta were used as immunogen.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LAMC1
Introduction
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. LAMC1 is the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of LAMC1 also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. Among its related pathways are ERK Signaling and Focal Adhesion.
Entrez Gene ID
3915
UniProt ID
P11047
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Biological Process
Animal organ morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Cell adhesionManual Assertion Based On ExperimentIDA:HGNC-UCL
Cell migrationManual Assertion Based On ExperimentIMP:HGNC-UCL
Endoderm developmentManual Assertion Based On ExperimentTAS:ProtInc
Extracellular matrix disassemblyManual Assertion Based On ExperimentIMP:HGNC-UCL
Hemidesmosome assemblyManual Assertion Based On ExperimentIMP:HGNC-UCL
Maintenance of blood-brain barrier1 PublicationNAS:ARUK-UCL
Positive regulation of epithelial cell proliferationManual Assertion Based On ExperimentTAS:HGNC-UCL
Protein-containing complex assemblyManual Assertion Based On ExperimentIDA:HGNC-UCL
Substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIDA:BHF-UCL
Tissue developmentManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane
More Infomation

Bai, J., Zheng, A., Ha, Y., Xu, X., Yu, Y., Lu, Y., ... & Jie, W. (2022). Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma. Frontiers in Molecular Biosciences, 9, 988777.

Wang, Z., Jiao, P., Zhong, Y., Ji, H., Zhang, Y., Song, H., ... & Wu, H. (2022). The endoplasmic reticulum-stressed head and neck squamous cell carcinoma cells induced exosomal miR-424-5p inhibits angiogenesis and migration of humanumbilical vein endothelial cells through LAMC1-mediated Wnt/β-catenin signaling pathway. Cell Transplantation, 31, 09636897221083549.

Fang, Y., Dou, R., Huang, S., Han, L., Fu, H., Yang, C., ... & Xiong, B. (2022). LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis. International Journal of Biological Sciences, 18(7), 3082.

Wang, Y. Q., Huang, G., Chen, J., Cao, H., & Xu, W. T. (2021). LncRNA SNHG6 promotes breast cancer progression and epithelial-mesenchymal transition via miR-543/LAMC1 axis. Breast Cancer Research and Treatment, 188, 1-14.

Fang, L., Che, Y., Zhang, C., Huang, J., Lei, Y., Lu, Z., ... & He, J. (2021). LAMC1 upregulation via TGFβ induces inflammatory cancer‐associated fibroblasts in esophageal squamous cell carcinoma via NF‐κB–CXCL1–STAT3. Molecular oncology, 15(11), 3125-3146.

Zhi-Rong, H. A. N., Jiang, X. L., & Wen-Chuan, F. A. N. (2021). LAMC1 is related to the poor prognosis of patients with gastric cancer and facilitates cancer cell malignancies. Neoplasma, 68(4).

Kunitomi, H., Kobayashi, Y., Wu, R. C., Takeda, T., Tominaga, E., Banno, K., & Aoki, D. (2020). LAMC1 is a prognostic factor and a potential therapeutic target in endometrial cancer. Journal of gynecologic oncology, 31(2).

Liu, J., Liu, D., Yang, Z., & Yang, Z. (2019). High LAMC1 expression in glioma is associated with poor prognosis. OncoTargets and therapy, 12, 4253.

Ye, G., Qin, Y., Wang, S., Pan, D., Xu, S., Wu, C., ... & Shen, H. (2019). Lamc1 promotes the Warburg effect in hepatocellular carcinoma cells by regulating PKM2 expression through AKT pathway. Cancer biology & therapy, 20(5), 711-719.

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For research use only. Not intended for any clinical use.

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