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Mouse Anti-KRT8 Recombinant Antibody (B22.1) (CBMAB-K2028-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Rat, Rabbit, Cattle, Chicken, Pig, Sheep, Frog
Clone
B22.1
Antibody Isotype
IgG1, κ
Application
IHC-P, IF/ICC, FC

Basic Information

Immunogen
Cytoskeletal preparation from HeLa cells
Specificity
Human, Rat, Rabbit, Cattle, Chicken, Pig, Sheep, Frog
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.05% BSA
Preservative
0.05% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Keratin 8
Introduction
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Entrez Gene ID
Human3856
Cattle281269
Rat25626
Rabbit100348891
Chicken426896
Pig100152077
Sheep101110495
UniProt ID
HumanP05787
CattleP05786
RatQ10758
ChickenA0A1L1RIW5
PigA0A287ARG4
SheepW5Q5N9
Alternative Names
Keratin 8; Type-II Keratin Kb8; Keratin 8, Type II; Cytokeratin-8; CK-8; CYK8; K8;
Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Cellular Location
Cytoplasm 2 Publications
Nucleus, nucleoplasm By Similarity
Nucleus matrix
Involvement in disease
Cirrhosis (CIRRH):
A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension.
PTM
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
More Infomation

Wang, D., Shang, Q., Mao, J., Gao, C., Wang, J., Wang, D., ... & Yang, L. (2023). Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration. Autophagy, 1-19.

Xiong, F., Guo, T., Wang, X., Wu, G., Liu, W., Wang, Q., ... & Chen, Y. (2022). Keratin 8 Is an Inflammation-Induced and Prognosis-Related Marker for Pancreatic Adenocarcinoma. Disease Markers, 2022.

Son, S., Baek, A., Lee, J. H., & Kim, D. E. (2022). Autophagosome–lysosome fusion is facilitated by plectin-stabilized actin and keratin 8 during macroautophagic process. Cellular and Molecular Life Sciences, 79(2), 95.

Baek, A., Son, S., Baek, Y. M., & Kim, D. E. (2021). KRT8 (keratin 8) attenuates necrotic cell death by facilitating mitochondrial fission-mediated mitophagy through interaction with PLEC (plectin). Autophagy, 17(12), 3939-3956.

Albaret, M. A., Paré, A., Malet, L., De Souza, G., Lavergne, E., Goga, D., ... & Saintigny, P. (2021). Combined proteomic and transcriptomic approaches reveal externalized keratin 8 as a potential therapeutic target involved in invasiveness of head and neck cancers. Translational Oncology, 14(1), 100878.

Miao, Q., Xu, Y., Yin, H., Zhang, H., & Ye, J. (2020). KRT8 phosphorylation regulates the epithelial‐mesenchymal transition in retinal pigment epithelial cells through autophagy modulation. Journal of Cellular and Molecular Medicine, 24(5), 3217-3228.

Golob-Schwarzl, N., Bettermann, K., Mehta, A. K., Kessler, S. M., Unterluggauer, J., Krassnig, S., ... & Haybaeck, J. (2019). High keratin 8/18 ratio predicts aggressive hepatocellular cancer phenotype. Translational oncology, 12(2), 256-268.

Xie, L., Dang, Y., Guo, J., Sun, X., Xie, T., Zhang, L., ... & Guo, X. (2019). High KRT8 expression independently predicts poor prognosis for lung adenocarcinoma patients. Genes, 10(1), 36.

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For research use only. Not intended for any clinical use.

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