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Rabbit Anti-ISL1 Monoclonal Antibody (EP4182) (CBMAB-1363-YC)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Chicken
Clone
EP4182
Antibody Isotype
IgG
Application
IP, FC, ICC, IF, WB

Basic Information

Immunogen
Synthetic peptide corresponding to Human Islet 1 aa 250 to the C-terminus.
Specificity
Human, Mouse, Chicken
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ISL LIM Homeobox 1
Introduction
Islet 1 is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
Entrez Gene ID
Human3670
Chicken396383
Mouse16392
UniProt ID
HumanP61371
ChickenP50211
MouseP61372
Alternative Names
Isl-1; ISLET1
Function
DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Biological Process
Atrial septum morphogenesisISS:BHF-UCL
Axon regenerationIEA:Ensembl
AxonogenesisManual Assertion Based On ExperimentIBA:GO_Central
Cardiac cell fate determinationManual Assertion Based On ExperimentIDA:BHF-UCL
Cardiac muscle cell myoblast differentiationIEA:Ensembl
Cardiac right ventricle morphogenesisISS:BHF-UCL
Cellular response to glucocorticoid stimulusISS:BHF-UCL
Endocardial cushion morphogenesisISS:BHF-UCL
Heart developmentISS:UniProtKB
InnervationISS:BHF-UCL
Mesenchymal cell differentiationISS:BHF-UCL
Negative regulation of canonical Wnt signaling pathwayIEA:Ensembl
Negative regulation of epithelial cell proliferationIEA:Ensembl
Negative regulation of inflammatory responseISS:BHF-UCL
Negative regulation of intracellular estrogen receptor signaling pathwayISS:BHF-UCL
Negative regulation of neuron apoptotic processISS:BHF-UCL
Negative regulation of neuron differentiationIEA:Ensembl
Negative regulation of protein-containing complex assemblyISS:BHF-UCL
Negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Neural crest cell migrationIEA:Ensembl
Neuron fate specificationISS:BHF-UCL
Outflow tract morphogenesisISS:BHF-UCL
Outflow tract septum morphogenesisISS:BHF-UCL
Pancreas developmentISS:BHF-UCL
Peripheral nervous system neuron axonogenesisISS:BHF-UCL
Pharyngeal system developmentISS:BHF-UCL
Pituitary gland developmentIEA:Ensembl
Positive regulation of angiogenesisISS:BHF-UCL
Positive regulation of cell differentiationISS:UniProtKB
Positive regulation of cell population proliferationIEA:Ensembl
Positive regulation of DNA bindingIEA:Ensembl
Positive regulation of epithelial to mesenchymal transitionIEA:Ensembl
Positive regulation of granulocyte colony-stimulating factor productionISS:BHF-UCL
Positive regulation of granulocyte macrophage colony-stimulating factor productionISS:BHF-UCL
Positive regulation of histone acetylationIEA:Ensembl
Positive regulation of insulin secretion1 PublicationIC:BHF-UCL
Positive regulation of interferon-gamma productionISS:BHF-UCL
Positive regulation of interleukin-1 alpha productionISS:BHF-UCL
Positive regulation of interleukin-1 beta productionISS:BHF-UCL
Positive regulation of interleukin-12 productionISS:BHF-UCL
Positive regulation of interleukin-6 productionISS:BHF-UCL
Positive regulation of macrophage colony-stimulating factor productionISS:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIGI:ParkinsonsUK-UCL
Positive regulation of tumor necrosis factor productionISS:BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT proteinIEA:Ensembl
Positive regulation of vascular endothelial growth factor productionISS:BHF-UCL
Regulation of heart rate by cardiac conductionISS:BHF-UCL
Regulation of secondary heart field cardioblast proliferationIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Retinal ganglion cell axon guidanceIEA:Ensembl
Secondary heart field specificationManual Assertion Based On ExperimentIMP:BHF-UCL
Sensory system developmentISS:BHF-UCL
Sinoatrial node cell developmentISS:BHF-UCL
Spinal cord motor neuron cell fate specificationIEA:Ensembl
Spinal cord motor neuron differentiationISS:BHF-UCL
Trigeminal nerve developmentISS:BHF-UCL
Ventricular cardiac muscle tissue morphogenesisISS:BHF-UCL
Visceral motor neuron differentiationIEA:Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated.
More Infomation

Yasuoka, Y., & Taira, M. (2021). LIM homeodomain proteins and associated partners: Then and now. Current topics in developmental biology, 145, 113-166.

Akhir, M. K. A. M., Choy, C. S., Abdullah, M. A., Abd Ghani, F., Veerakumarasivam, A., & Hussin, H. (2020). The role of ISL1 and LHX5 LIM homeobox genes in bladder tumourigenesis. The Malaysian journal of medical sciences: MJMS, 27(1), 37.

Juhlin, C. C., Zedenius, J., & Höög, A. (2020). Clinical routine application of the second-generation neuroendocrine markers ISL1, INSM1, and secretagogin in neuroendocrine neoplasia: staining outcomes and potential clues for determining tumor origin. Endocrine Pathology, 31, 401-410.

Su, T., Liu, H., Zhang, D., Xu, G., Liu, J., Evans, S. M., ... & Cui, S. (2019). LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh. Cell Death & Disease, 10(10), 713.

Zhao, L. H., Chen, C., Mao, C. Y., Xiao, H., Fu, P., Xiao, H. L., & Wang, G. (2019). Value of SATB2, ISL1, and TTF1 to differentiate rectal from other gastrointestinal and lung well-differentiated neuroendocrine tumors. Pathology-Research and Practice, 215(7), 152448.

Shao, W., Szeto, V., Song, Z., Tian, L., Feng, Z. P., Nostro, M. C., & Jin, T. (2018). The LIM homeodomain protein ISL1 mediates the function of TCF7L2 in pancreatic beta cells. Journal of Molecular Endocrinology, 61(1), 1-12.

Zhang, J., Qiu, J., Zhou, Y., Wang, Y., Li, H., Zhang, T., ... & Cui, S. (2018). LIM homeobox transcription factor Isl1 is required for melatonin synthesis in the pig pineal gland. Journal of pineal research, 65(1), e12481.

Sharma, A., Dakal, T. C., Ludwig, M., Fröhlich, H., Mathur, R., & Reutter, H. (2018). Towards a central role of ISL1 in the bladder exstrophy–epispadias complex (BEEC): computational characterization of genetic variants and structural modelling. Genes, 9(12), 609.

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For research use only. Not intended for any clinical use.

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