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Rabbit Anti-IQGAP1 Recombinant Antibody (EG1665) (CBMAB-EN1993-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EG1665
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:20000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from N-terminal of human IQGAP1.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
IQ motif containing GTPase activating protein 1
Introduction
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
Entrez Gene ID
Human8826
Mouse29875
UniProt ID
HumanP46940
MouseQ9JKF1
Alternative Names
SAR1; p195; HUMORFA01
Function
Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth (PubMed:15695813).
May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed:20883816).
Biological Process
Cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Cellular response to calcium ionManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular response to epidermal growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to platelet-derived growth factor stimulusIEA:Ensembl
Epidermal growth factor receptor signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Fibroblast growth factor receptor signaling pathwayIEA:Ensembl
Fibroblast migrationIEA:Ensembl
Glomerular visceral epithelial cell developmentISS:UniProtKB
Negative regulation of dephosphorylationIEA:Ensembl
Neuron projection extensionManual Assertion Based On ExperimentIMP:UniProtKB
Platelet-derived growth factor receptor signaling pathwayIEA:Ensembl
Positive regulation of MAPK cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of cytokine productionIEA:Ensembl
Regulation of GTPase activityIEA:InterPro
Regulation of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cell membrane; Nucleus; Cytoplasm. Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816).
PTM
Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
More Infomation

Hoeprich, G. J., Sinclair, A. N., Shekhar, S., & Goode, B. L. (2022). Single-molecule imaging of IQGAP1 regulating actin filament dynamics. Molecular biology of the cell, 33(1), ar2.

Zhang, B., Cheng, X., Zhan, S., Jin, X., & Liu, T. (2021). MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation. Molecular Oncology, 15(11), 3062-3075.

Wei, T., & Lambert, P. F. (2021). Role of IQGAP1 in Carcinogenesis. Cancers, 13(16), 3940.

Peng, X., Wang, T., Gao, H., Yue, X., Bian, W., Mei, J., & Zhang, Y. (2021). The interplay between IQGAP1 and small GTPases in cancer metastasis. Biomedicine & Pharmacotherapy, 135, 111243.

Wei, T., Choi, S., Buehler, D., Lee, D., Ward-Shaw, E., Anderson, R. A., & Lambert, P. F. (2021). Role of IQGAP1 in papillomavirus-associated head and neck tumorigenesis. Cancers, 13(9), 2276.

Hebert, J. D., Tian, C., Lamar, J. M., Rickelt, S., Abbruzzese, G., Liu, X., & Hynes, R. O. (2020). The scaffold protein IQGAP1 is crucial for extravasation and metastasis. Scientific Reports, 10(1), 2439.

Chuang, H. C., Chang, C. C., Teng, C. F., Hsueh, C. H., Chiu, L. L., Hsu, P. M., ... & Tan, T. H. (2019). MAP4K3/GLK promotes lung cancer metastasis by phosphorylating and activating IQGAP1. Cancer Research, 79(19), 4978-4993.

Hu, W., Wang, Z., Zhang, S., Lu, X., Wu, J., Yu, K., ... & Jiang, C. (2019). IQGAP1 promotes pancreatic cancer progression and epithelial-mesenchymal transition (EMT) through Wnt/β-catenin signaling. Scientific reports, 9(1), 7539.

Chen, M., Choi, S., Jung, O., Wen, T., Baum, C., Thapa, N., ... & Anderson, R. A. (2019). The specificity of EGF-stimulated IQGAP1 scaffold towards the PI3K-Akt pathway is defined by the IQ3 motif. Scientific reports, 9(1), 9126.

Tanos, B. E., Yeaman, C., & Rodriguez-Boulan, E. (2018). An emerging role for IQGAP1 in tight junction control. Small GTPases, 9(5), 375-383.

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For research use only. Not intended for any clinical use.

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