Rabbit Anti-HDAC6 Recombinant Antibody (D21B10) (CBMAB-CP0925-LY)

Rabbit

Human, Mouse, Rat

D21B10

IgG

WB, IP

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro681 of human HDAC6 protein.

Human, Mouse, Rat

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

100 µg/ml BSA, 50% glycerol

0.02% sodium azide

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

HDAC6

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Histone Deacetylase 6; Protein Phosphatase 1; Regulatory Subunit 90; EC 3.5.1.98; HD6; KIAA0901; PPP1R90; CPBHM; JM21;

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10220385).

Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10220385).

Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10220385).

In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:12024216, PubMed:20308065).

Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141).

Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532).

In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (PubMed:17846173).

Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:17846173).

Aggresome assembly Source: BHF-UCL
Autophagy Source: UniProtKB-KW
Axonal transport of mitochondrion Source: ARUK-UCL
Cellular response to hydrogen peroxide Source: BHF-UCL
Cellular response to misfolded protein Source: Ensembl
Cellular response to topologically incorrect protein Source: BHF-UCL
Cilium assembly Source: Reactome
Collateral sprouting Source: Ensembl
Dendritic spine morphogenesis Source: Ensembl
Histone deacetylation Source: BHF-UCL
Hsp90 deacetylation Source: Ensembl
Intracellular protein transport Source: BHF-UCL
Lysosome localization Source: BHF-UCL
Negative regulation of hydrogen peroxide metabolic process Source: BHF-UCL
Negative regulation of microtubule depolymerization Source: Ensembl
Negative regulation of oxidoreductase activity Source: BHF-UCL
Negative regulation of protein-containing complex assembly Source: ARUK-UCL
Negative regulation of protein-containing complex disassembly Source: BHF-UCL
Negative regulation of proteolysis Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Peptidyl-lysine deacetylation Source: BHF-UCL
Polyubiquitinated misfolded protein transport Source: BHF-UCL
Positive regulation of epithelial cell migration Source: BHF-UCL
Positive regulation of hydrogen peroxide-mediated programmed cell death Source: BHF-UCL
Positive regulation of peptidyl-serine phosphorylation Source: ARUK-UCL
Positive regulation of signaling receptor activity Source: BHF-UCL
Protein-containing complex disassembly Source: Ensembl
Protein deacetylation Source: UniProtKB
Protein destabilization Source: Ensembl
Protein polyubiquitination Source: Ensembl
Protein quality control for misfolded or incompletely synthesized proteins Source: BHF-UCL
Regulation of androgen receptor signaling pathway Source: BHF-UCL
Regulation of autophagy Source: ParkinsonsUK-UCL
Regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
Regulation of establishment of protein localization Source: Ensembl
Regulation of fat cell differentiation Source: Ensembl
Regulation of gene expression, epigenetic Source: UniProtKB
Regulation of macroautophagy Source: BHF-UCL
Regulation of microtubule-based movement Source: BHF-UCL
Regulation of protein stability Source: ParkinsonsUK-UCL
Response to growth factor Source: BHF-UCL
Response to misfolded protein Source: BHF-UCL
Tubulin deacetylation Source: UniProtKB
Ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: Ensembl

Cytoplasm; Nucleus; Cytoskeleton; Perikaryon; Dendrite; Axon. It is mainly cytoplasmic, where it is associated with microtubules.

Chondrodysplasia with platyspondyly, distinctive brachydactyly, hydrocephaly, and microphthalmia (CDP-PBHM):
A disease characterized by chondrodysplasia, severe platyspondyly, hydrocephaly, and facial features with microphthalmia. Bone abnormalities include a distinctive metaphyseal cupping of the metacarpals, metatarsals, and phalanges. Affected females show a milder phenotype with small stature, sometimes associated with body asymmetry and mild mental retardation.

Phosphorylated by AURKA.
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.