Mouse Anti-HAUS1 Recombinant Antibody (1E3) (CBMAB-A1159-LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1E3

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

CCDC5 (NP_612452.1, 179 a.a. ~ 278 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

HAUS Augmin Like Complex Subunit 1

Entrez Gene ID

115106

UniProt ID

Q96CS2

Alternative Names

FLJ21094; FLJ40084; HEI-C; HsT1461

Function

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

Biological Process

Cell division Source: UniProtKB-KW
Centrosome cycle Source: UniProtKB
Spindle assembly Source: UniProtKB

Cellular Location

Centrosome; Spindle; Spindle pole; Cytoplasm. Localizes with the spindle poles in mitotic cells. In metaphase, localizes to the mitotic asters and is highly punctate on the microtubule array. During later stages of mitosis, remains on the spindle but is not present at the interzone, and is finally observed at the microtubule bundles proximal to the midbody, clearly excluded from the midbody. In contrast, does not colocalize with the tubulin cytoskeleton in interphase cells. In interphase, localized at the centrosome and diffusely in the cytoplasm. Localizes to mitotic spindle microtubules.

More Infomation

References

Ustinova, K., Ruhnow, F., Gili, M., & Surrey, T. (2023). Microtubule binding of the human HAUS complex is directly controlled by importins and Ran-GTP. bioRxiv, 2023-02.

Manka, S. W. (2023). Structural insights into how augmin augments the mitotic spindle. nature communications, 14(1), 2073.

Yao, Q., Ge, X., Lu, Z., Shi, J., Shen, J., & Chen, J. (2022). HAUS augmin-like complex subunit 1 influences tumour microenvironment and prognostic outcomes in glioma. Journal of Oncology, 2022.

Song, J. G., King, M. R., Zhang, R., Kadzik, R. S., Thawani, A., & Petry, S. (2018). Mechanism of how augmin directly targets the γ-tubulin ring complex to microtubules. Journal of Cell Biology, 217(7), 2417-2428.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-HAUS1 Recombinant Antibody (1E3)

Related Products

Mouse Anti-HAUS1 (AA 179-279) Recombinant Antibody (CBFYH-0718)

Mouse Anti-HAUS1 Recombinant Antibody (1E3)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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