Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-GSDMD Recombinant Antibody (3F12-1B2) (CBMAB-D2080-YC)


See all GSDMD antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F12-1B2
Antibody Isotype
IgG1
Application
WB, ELISA, IHC-P, ICC, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Gasdermin D
Introduction
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor.
Entrez Gene ID
79792
UniProt ID
P57764
Alternative Names
Gasdermin D; Gasdermin Domain-Containing Protein 1; Gasdermin Domain Containing 1; GSDMDC1; DFNA5L; Gasdermin-D; FKSG10; DF5L;
Function
Gasdermin-D:
Precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27281216).

This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:26375259, PubMed:27281216).

Gasdermin-D, N-terminal:
Promotes pyroptosis in response to microbial infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27418190, PubMed:28392147, PubMed:32820063).

Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1, CASP4 or CASP5 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190).

After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216, PubMed:29898893).

Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216, PubMed:29898893).

Exhibits bactericidal activity (PubMed:27281216).

Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216).

Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity).

Also active in response to MAP3K7/TAK1 inactivation by Yersinia toxin YopJ, which triggers cleavage by CASP8 and subsequent activation (By similarity).

Strongly binds to bacterial and mitochondrial lipids, including cardiolipin (PubMed:27281216).

Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
Biological Process
Defense response to bacterium Source: GO_Central
Defense response to Gram-negative bacterium Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Neutrophil degranulation Source: Reactome
Pore complex assembly Source: UniProtKB
Pore formation in membrane of other organism Source: UniProtKB
Positive regulation of interleukin-1 beta production Source: UniProtKB
Protein homooligomerization Source: UniProtKB
Pyroptosis Source: UniProtKB
Cellular Location
Gasdermin-D: Cytosol; Inflammasome. In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor.
Gasdermin-D, N-terminal: Secreted; Cell membrane. Released in the extracellular milieu following pyroptosis.
Gasdermin-D, C-terminal: Cytosol
PTM
Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).
Gasdermin-D:
Succination of Cys-191 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits processing by caspases, and ability to initiate pyroptosis (PubMed:32820063). Succination modification is catalyzed by a non-enzymatic reaction caused by an accumulation of fumarate (PubMed:32820063).
More Infomation

Xie, W. J., Xia, S., Warshel, A., & Wu, H. (2022). Electrostatic influence on IL-1 transport through the GSDMD pore. Proceedings of the National Academy of Sciences, 119(6), e2120287119.

Liu, Y., Xue, N., Zhang, B., Lv, H., & Li, S. (2022). Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway. Biomolecules, 12(7), 927.

Jiang, S., Zhang, H., Li, X., Yi, B., Huang, L., Hu, Z., ... & Zhang, W. (2021). Vitamin D/VDR attenuate cisplatin-induced AKI by down-regulating NLRP3/Caspase-1/GSDMD pyroptosis pathway. The Journal of Steroid Biochemistry and Molecular Biology, 206, 105789.

Zhang, J., Dai, Y., Yang, Y., & Xu, J. (2021). Calcitriol alleviates hyperosmotic stress-induced corneal epithelial cell damage via inhibiting the NLRP3–ASC–caspase-1–GSDMD pyroptosis pathway in dry eye disease. Journal of Inflammation Research, 14, 2955.

Yan, H., Luo, B., Wu, X., Guan, F., Yu, X., Zhao, L., ... & Yuan, J. (2021). Cisplatin induces pyroptosis via activation of MEG3/NLRP3/caspase-1/GSDMD pathway in triple-negative breast cancer. International Journal of Biological Sciences, 17(10), 2606.

Li, W., Deng, M., Loughran, P. A., Yang, M., Lin, M., Yang, C., ... & Scott, M. J. (2020). LPS induces active HMGB1 release from hepatocytes into exosomes through the coordinated activities of TLR4 and caspase-11/GSDMD signaling. Frontiers in Immunology, 11, 229.

Yang, Y., Liu, P. Y., Bao, W., Chen, S. J., Wu, F. S., & Zhu, P. Y. (2020). Hydrogen inhibits endometrial cancer growth via a ROS/NLRP3/caspase-1/GSDMD-mediated pyroptotic pathway. BMC cancer, 20, 1-19.

Schwarzer, R., Jiao, H., Wachsmuth, L., Tresch, A., & Pasparakis, M. (2020). FADD and caspase-8 regulate gut homeostasis and inflammation by controlling MLKL-and GSDMD-mediated death of intestinal epithelial cells. Immunity, 52(6), 978-993.

Karmakar, M., Minns, M., Greenberg, E. N., Diaz-Aponte, J., Pestonjamasp, K., Johnson, J. L., ... & Pearlman, E. (2020). N-GSDMD trafficking to neutrophil organelles facilitates IL-1β release independently of plasma membrane pores and pyroptosis. Nature communications, 11(1), 2212.

Pandeya, A., Li, L., Li, Z., & Wei, Y. (2019). Gasdermin D (GSDMD) as a new target for the treatment of infection. Medchemcomm, 10(5), 660-667.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-GSDMD Recombinant Antibody (3F12-1B2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry