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Mouse Anti-GPRC5B Recombinant Antibody (1H1) (CBMAB-G4687-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1H1
Antibody Isotype
IgG2a
Application
FC, CyTOF-ready

Basic Information

Immunogen
NS0 mouse myeloma cell line transfected with human GPRC5B
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G Protein-Coupled Receptor Class C Group 5 Member B
Introduction
This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Entrez Gene ID
51704
UniProt ID
Q9NZH0
Alternative Names
G Protein-Coupled Receptor Class C Group 5 Member B; G Protein-Coupled Receptor; Family C; Group 1; Member B; Retinoic Acid-Induced Gene 2 Protein; RAIG-2; RAIG2; G Protein-Coupled Receptor; Family C; Group 5; Member B;
Function
Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Biological Process
Positive regulation of canonical Wnt signaling pathway Source: CAFA
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
Positive regulation of inflammatory response Source: CAFA
Positive regulation of macrophage cytokine production Source: CAFA
Positive regulation of neuron differentiation Source: CAFA
Positive regulation of protein tyrosine kinase activity Source: CAFA
Cellular Location
Cell membrane; Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
Topology
Extracellular: 29-56
Helical: 57-77
Cytoplasmic: 78-94
Helical: 95-115
Extracellular: 116-126
Helical: 127-147
Cytoplasmic: 148-162
Helical: 163-183
Extracellular: 184-199
Helical: 200-220
Cytoplasmic: 221-234
Helical: 235-255
Extracellular: 256-271
Helical: 272-292
Cytoplasmic: 293-403
More Infomation

Fu, W., Franchini, L., & Orlandi, C. (2022). Comprehensive Spatial Profile of the Orphan G Protein Coupled Receptor GPRC5B Expression in Mouse Brain. Frontiers in Neuroscience, 16, 891544.

Xu, W., Nelson-Maney, N. P., Bálint, L., Kwon, H. B., Davis, R. B., Dy, D. C., ... & Caron, K. M. (2022). Orphan G-Protein Coupled Receptor GPRC5B Is Critical for Lymphatic Development. International Journal of Molecular Sciences, 23(10), 5712.

Alonso-Gardón, M., Elorza-Vidal, X., Castellanos, A., La Sala, G., Armand-Ugon, M., Gilbert, A., ... & Estévez, R. (2021). Identification of the GlialCAM interactome: the G protein-coupled receptors GPRC5B and GPR37L1 modulate megalencephalic leukoencephalopathy proteins. Human Molecular Genetics, 30(17), 1649-1665.

Hirabayashi, Y., & Kim, Y. J. (2020). Roles of GPRC5 family proteins: focusing on GPRC5B and lipid-mediated signalling. The journal of biochemistry, 167(6), 541-547.

Carvalho, J., Chennupati, R., Li, R., Günther, S., Kaur, H., Zhao, W., ... & Wettschureck, N. (2020). Orphan G protein–coupled receptor GPRC5B controls smooth muscle contractility and differentiation by inhibiting prostacyclin receptor signaling. Circulation, 141(14), 1168-1183.

Zambrano, S., Möller-Hackbarth, K., Li, X., Rodriguez, P. Q., Charrin, E., Schwarz, A., ... & Patrakka, J. (2019). GPRC5B modulates inflammatory response in glomerular diseases via NF-κB pathway. Journal of the American Society of Nephrology, 30(9), 1573-1586.

von Samson-Himmelstjerna, F. A., Freundt, G., Nitz, J. T., Stelter, F., Luedde, M., Wieland, T., ... & Hippe, H. J. (2019). The orphan receptor GPRC5B modulates inflammatory and fibrotic pathways in cardiac fibroblasts and mice hearts. Biochemical and biophysical research communications, 514(4), 1198-1203.

Kim, Y. J., Greimel, P., & Hirabayashi, Y. (2018). GPRC5B-mediated sphingomyelin synthase 2 phosphorylation plays a critical role in insulin resistance. IScience, 8, 250-266.

Sano, T., Kohyama-Koganeya, A., Kinoshita, M. O., Tatsukawa, T., Shimizu, C., Oshima, E., ... & Hirabayashi, Y. (2018). Loss of GPRC5B impairs synapse formation of Purkinje cells with cerebellar nuclear neurons and disrupts cerebellar synaptic plasticity and motor learning. Neuroscience research, 136, 33-47.

Atanes, P., Ruz-Maldonado, I., Hawkes, R., Liu, B., Persaud, S. J., & Amisten, S. (2018). Identifying signalling pathways regulated by GPRC5B in β-Cells by CRISPR-Cas9-mediated genome editing. Cellular Physiology and Biochemistry, 45(2), 656-666.

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For research use only. Not intended for any clinical use.

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