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Rabbit Anti-GPRC5B Recombinant Antibody (BA0082) (CBMAB-0353CQ)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0082
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Human GPCR GPRC5B aa 1-100
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
G Protein-Coupled Receptor Class C Group 5 Member B
Introduction
G protein-coupled receptors (GPCRs) share a common structure of 7 transmembrane domains, an extracellular N-terminal domain, an intracellular C-terminal domain, and several conserved residues. Family C of the GPCR superfamily, which includes GPRC5B, consists of metabotropic glutamate receptor-like proteins. This gene encodes a member of the type 3 G protein-coupled receptor family. Using STS analysis, Robbins et al. (2000) mapped the GPRC5B gene to 16p12.3-p12.1. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human51704
Mouse64297
Rat293546
UniProt ID
HumanQ9NZH0
MouseQ923Z0
RatD4A3F5
Alternative Names
G Protein-Coupled Receptor Class C Group 5 Member B; G Protein-Coupled Receptor, Class C, Group 5, Member B; G Protein-Coupled Receptor, Family C, Group 1, Member B; Retinoic Acid-Induced Gene 2 Protein; RAIG-2; RAIG2; G Protein-Coupled Receptor, Family C, Group 5, Member B; Retinoic Acid Responsive Gene Protein; A-69G12.1
Function
Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Biological Process
Positive regulation of canonical Wnt signaling pathway Source: CAFA
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
Positive regulation of inflammatory response Source: CAFA
Positive regulation of macrophage cytokine production Source: CAFA
Positive regulation of neuron differentiation Source: CAFA
Positive regulation of protein tyrosine kinase activity Source: CAFA
Cellular Location
Cell membrane; Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
Topology
Extracellular: 29-56
Helical: 57-77
Cytoplasmic: 78-94
Helical: 95-115
Extracellular: 116-126
Helical: 127-147
Cytoplasmic: 148-162
Helical: 163-183
Extracellular: 184-199
Helical: 200-220
Cytoplasmic: 221-234
Helical: 235-255
Extracellular: 256-271
Helical: 272-292
Cytoplasmic: 293-403
More Infomation

Fu, W., Franchini, L., & Orlandi, C. (2022). Comprehensive Spatial Profile of the Orphan G Protein Coupled Receptor GPRC5B Expression in Mouse Brain. Frontiers in Neuroscience, 16, 891544.

Xu, W., Nelson-Maney, N. P., Bálint, L., Kwon, H. B., Davis, R. B., Dy, D. C., ... & Caron, K. M. (2022). Orphan G-Protein Coupled Receptor GPRC5B Is Critical for Lymphatic Development. International Journal of Molecular Sciences, 23(10), 5712.

Alonso-Gardón, M., Elorza-Vidal, X., Castellanos, A., La Sala, G., Armand-Ugon, M., Gilbert, A., ... & Estévez, R. (2021). Identification of the GlialCAM interactome: the G protein-coupled receptors GPRC5B and GPR37L1 modulate megalencephalic leukoencephalopathy proteins. Human Molecular Genetics, 30(17), 1649-1665.

Hirabayashi, Y., & Kim, Y. J. (2020). Roles of GPRC5 family proteins: focusing on GPRC5B and lipid-mediated signalling. The journal of biochemistry, 167(6), 541-547.

Carvalho, J., Chennupati, R., Li, R., Günther, S., Kaur, H., Zhao, W., ... & Wettschureck, N. (2020). Orphan G protein–coupled receptor GPRC5B controls smooth muscle contractility and differentiation by inhibiting prostacyclin receptor signaling. Circulation, 141(14), 1168-1183.

Zambrano, S., Möller-Hackbarth, K., Li, X., Rodriguez, P. Q., Charrin, E., Schwarz, A., ... & Patrakka, J. (2019). GPRC5B modulates inflammatory response in glomerular diseases via NF-κB pathway. Journal of the American Society of Nephrology, 30(9), 1573-1586.

von Samson-Himmelstjerna, F. A., Freundt, G., Nitz, J. T., Stelter, F., Luedde, M., Wieland, T., ... & Hippe, H. J. (2019). The orphan receptor GPRC5B modulates inflammatory and fibrotic pathways in cardiac fibroblasts and mice hearts. Biochemical and biophysical research communications, 514(4), 1198-1203.

Kim, Y. J., Greimel, P., & Hirabayashi, Y. (2018). GPRC5B-mediated sphingomyelin synthase 2 phosphorylation plays a critical role in insulin resistance. IScience, 8, 250-266.

Sano, T., Kohyama-Koganeya, A., Kinoshita, M. O., Tatsukawa, T., Shimizu, C., Oshima, E., ... & Hirabayashi, Y. (2018). Loss of GPRC5B impairs synapse formation of Purkinje cells with cerebellar nuclear neurons and disrupts cerebellar synaptic plasticity and motor learning. Neuroscience research, 136, 33-47.

Atanes, P., Ruz-Maldonado, I., Hawkes, R., Liu, B., Persaud, S. J., & Amisten, S. (2018). Identifying signalling pathways regulated by GPRC5B in β-Cells by CRISPR-Cas9-mediated genome editing. Cellular Physiology and Biochemistry, 45(2), 656-666.

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For research use only. Not intended for any clinical use.

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