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Rabbit Anti-GPRC5A Recombinant Antibody (EG1412) (CBMAB-EN1691-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1412
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:10000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPRC5A.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor class C group 5 member A
Introduction
This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation.
Entrez Gene ID
Human9052
Mouse232431
Rat312790
UniProt ID
HumanQ8NFJ5
MouseQ8BHL4
RatA1A5R2
Alternative Names
RAI3; TIG1; RAIG1; GPCR5A; PEIG-1
Function
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity).

May act as a lung tumor suppressor (PubMed:18000218).
Biological Process
Negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cell membrane; Cytoplasmic vesicle membrane. Localized in perinuclear vesicles, probably Golgi-associated vesicles.
Topology
Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-68
Helical: 69-89
Extracellular: 90-97
Helical: 98-118
Cytoplasmic: 119-129
Helical: 130-150
Extracellular: 151-176
Helical: 177-197
Cytoplasmic: 198-212
Helical: 213-233
Extracellular: 234-247
Helical: 248-268
Cytoplasmic: 269-357
PTM
Phosphorylated in two conserved double-tyrosine motifs, TYR-317/TYR-320 and TYR-347/TYR-350, by EGFR; leading to inactivation of the tumor suppressive function of GPRC5A in lung cancer cells. TYR-317 and TYR-320 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation.
More Infomation

Wei, R., Qi, G., Zeng, Z., Shen, N., Wang, Z., Shen, H., ... & Wang, C. (2021). IMUP and GPRC5A: two newly identified risk score indicators in pancreatic ductal adenocarcinoma. Cancer Cell International, 21, 1-19.

Moyano‐Galceran, L., Pietilä, E. A., Turunen, S. P., Corvigno, S., Hjerpe, E., Bulanova, D., ... & Lehti, K. (2020). Adaptive RSK‐EphA2‐GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer. EMBO molecular medicine, 12(4), e11177.

Sawada, Y., Kikugawa, T., Iio, H., Sakakibara, I., Yoshida, S., Ikedo, A., ... & Imai, Y. (2020). GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer. International Journal of Cancer, 146(5), 1369-1382.

Wang, T., Jing, B., Xu, D., Liao, Y., Song, H., Sun, B., ... & Deng, J. (2020). PTGES/PGE2 signaling links immunosuppression and lung metastasis in Gprc5a-knockout mouse model. Oncogene, 39(15), 3179-3194.

Richter, L., Oberländer, V., & Schmidt, G. (2020). RhoA/C inhibits proliferation by inducing the synthesis of GPRC5A. Scientific Reports, 10(1), 12532.

Greenhough, A., Bagley, C., Heesom, K. J., Gurevich, D. B., Gay, D., Bond, M., ... & Williams, A. C. (2018). Cancer cell adaptation to hypoxia involves a HIF‐GPRC5A‐YAP axis. EMBO molecular medicine, 10(11), e8699.

Gu, C., Zhou, N., Wang, Z., Li, G., Kou, Y., Yu, S., ... & Tian, F. (2018). circGprc5a promoted bladder oncogenesis and metastasis through Gprc5a-targeting peptide. Molecular Therapy-Nucleic Acids, 13, 633-641.

Jiang, X., Xu, X., Wu, M., Guan, Z., Su, X., Chen, S., ... & Teng, L. (2018). GPRC5A: an emerging biomarker in human cancer. BioMed Research International, 2018.

Liu, B., Yang, H., Pilarsky, C., & Weber, G. F. (2018). The effect of GPRC5a on the proliferation, migration ability, chemotherapy resistance, and phosphorylation of GSK-3β in pancreatic cancer. International journal of molecular sciences, 19(7), 1870.

Ma, X., Schwarz, A., Sevilla, S. Z., Levin, A., Hultenby, K., Wernerson, A., ... & Patrakka, J. (2018). Depletion of Gprc5a promotes development of diabetic nephropathy. Journal of the American Society of Nephrology, 29(6), 1679-1689.

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For research use only. Not intended for any clinical use.

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