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Mouse Anti-GPRC5A Recombinant Antibody (481906) (CBMAB-G0726-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
481906
Antibody Isotype
IgG2a
Application
FC

Basic Information

Immunogen
NS0 mouse myeloma cell line transfected with human GPRC5A, Met1-Ser357, Accession # Q8NFJ5
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor class C group 5 member A
Introduction
This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]
Entrez Gene ID
9052
UniProt ID
Q8NFJ5
Alternative Names
G Protein-Coupled Receptor Class C Group 5 Member A; G-Protein Coupled Receptor Family C Group 5 Member A; Retinoic Acid-Induced Gene 1 Protein; Phorbol Ester Induced Gene 1; Retinoic Acid Induced 3; GPCR5A; PEIG-1; RAIG1; RAI3;
Function
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity).

May act as a lung tumor suppressor (PubMed:18000218).
Biological Process
Negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cell membrane; Cytoplasmic vesicle membrane. Localized in perinuclear vesicles, probably Golgi-associated vesicles.
Topology
Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-68
Helical: 69-89
Extracellular: 90-97
Helical: 98-118
Cytoplasmic: 119-129
Helical: 130-150
Extracellular: 151-176
Helical: 177-197
Cytoplasmic: 198-212
Helical: 213-233
Extracellular: 234-247
Helical: 248-268
Cytoplasmic: 269-357
PTM
Phosphorylated in two conserved double-tyrosine motifs, TYR-317/TYR-320 and TYR-347/TYR-350, by EGFR; leading to inactivation of the tumor suppressive function of GPRC5A in lung cancer cells. TYR-317 and TYR-320 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation.
More Infomation

Wei, R., Qi, G., Zeng, Z., Shen, N., Wang, Z., Shen, H., ... & Wang, C. (2021). IMUP and GPRC5A: two newly identified risk score indicators in pancreatic ductal adenocarcinoma. Cancer Cell International, 21, 1-19.

Moyano‐Galceran, L., Pietilä, E. A., Turunen, S. P., Corvigno, S., Hjerpe, E., Bulanova, D., ... & Lehti, K. (2020). Adaptive RSK‐EphA2‐GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer. EMBO molecular medicine, 12(4), e11177.

Sawada, Y., Kikugawa, T., Iio, H., Sakakibara, I., Yoshida, S., Ikedo, A., ... & Imai, Y. (2020). GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer. International Journal of Cancer, 146(5), 1369-1382.

Wang, T., Jing, B., Xu, D., Liao, Y., Song, H., Sun, B., ... & Deng, J. (2020). PTGES/PGE2 signaling links immunosuppression and lung metastasis in Gprc5a-knockout mouse model. Oncogene, 39(15), 3179-3194.

Richter, L., Oberländer, V., & Schmidt, G. (2020). RhoA/C inhibits proliferation by inducing the synthesis of GPRC5A. Scientific Reports, 10(1), 12532.

Greenhough, A., Bagley, C., Heesom, K. J., Gurevich, D. B., Gay, D., Bond, M., ... & Williams, A. C. (2018). Cancer cell adaptation to hypoxia involves a HIF‐GPRC5A‐YAP axis. EMBO molecular medicine, 10(11), e8699.

Gu, C., Zhou, N., Wang, Z., Li, G., Kou, Y., Yu, S., ... & Tian, F. (2018). circGprc5a promoted bladder oncogenesis and metastasis through Gprc5a-targeting peptide. Molecular Therapy-Nucleic Acids, 13, 633-641.

Jiang, X., Xu, X., Wu, M., Guan, Z., Su, X., Chen, S., ... & Teng, L. (2018). GPRC5A: an emerging biomarker in human cancer. BioMed Research International, 2018.

Liu, B., Yang, H., Pilarsky, C., & Weber, G. F. (2018). The effect of GPRC5a on the proliferation, migration ability, chemotherapy resistance, and phosphorylation of GSK-3β in pancreatic cancer. International journal of molecular sciences, 19(7), 1870.

Ma, X., Schwarz, A., Sevilla, S. Z., Levin, A., Hultenby, K., Wernerson, A., ... & Patrakka, J. (2018). Depletion of Gprc5a promotes development of diabetic nephropathy. Journal of the American Society of Nephrology, 29(6), 1679-1689.

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For research use only. Not intended for any clinical use.

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