Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-FRZB Recombinant Antibody (4E5) (CBMAB-A3174-LY)


See all FRZB antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
4E5
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
FRZB (NP_001454, 102 a.a. ~ 190 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
frizzled-related protein
Entrez Gene ID
2487
UniProt ID
Q92765
Alternative Names
FRE; FRITZ; FRP-3; FRZB-1; FRZB-PEN; FRZB1; FZRB; SFRP3; SRFP3; hFIZ
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
Biological Process
Canonical Wnt signaling pathway Source: GO_Central
Cochlea morphogenesis Source: Ensembl
Convergent extension involved in organogenesis Source: Ensembl
Negative regulation of canonical Wnt signaling pathway Source: UniProtKB
Negative regulation of cartilage development Source: Ensembl
Negative regulation of cell development Source: Ensembl
Negative regulation of cell growth Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of hepatocyte differentiation Source: Ensembl
Negative regulation of Wnt signaling pathway Source: UniProtKB
Neural crest cell differentiation Source: Ensembl
Positive regulation of apoptotic process Source: MGI
Positive regulation of fat cell differentiation Source: MGI
Skeletal system development Source: ProtInc
Somite development Source: Ensembl
Cellular Location
Secreted
Involvement in disease
Osteoarthritis 1 (OS1):
A degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement.
More Infomation

Tuerlings, M., van Hoolwerff, M., van Bokkum, J. M., Suchiman, H. E. D., Lakenberg, N., Broekhuis, D., ... & Meulenbelt, I. (2022). Long non-coding RNA expression profiling of subchondral bone reveals AC005165. 1 modifying FRZB expression during osteoarthritis. Rheumatology, 61(7), 3023-3032.

Xu, R., Zhang, F., Lu, J., Wang, K., Pan, P., Sun, Y., & Zhang, Y. (2021). Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip. Aging (Albany NY), 13(8), 11281.

Huang, X., Zhong, L., van Helvoort, E., Lafeber, F., Mastbergen, S., Hendriks, J., ... & Karperien, M. (2021). The expressions of Dickkopf-related protein 1 and frizzled-related protein are negatively correlated to local inflammation and osteoarthritis severity. Cartilage, 12(4), 496-504.

Casas-Fraile, L., Cornelis, F. M., Costamagna, D., Rico, A., Duelen, R., Sampaolesi, M. M., ... & Sáenz, A. (2020). Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels. Orphanet Journal of Rare Diseases, 15(1), 1-18.

Kwan, T., Kazamel, M., Thoenes, K., Si, Y., Jiang, N., & King, P. H. (2020). Wnt antagonist FRZB is a muscle biomarker of denervation atrophy in amyotrophic lateral sclerosis. Scientific reports, 10(1), 16679.

Claudel, M., Jouzeau, J. Y., & Cailotto, F. (2019). Secreted Frizzled‐related proteins (sFRPs) in osteo‐articular diseases: much more than simple antagonists of Wnt signaling?. The FEBS Journal, 286(24), 4832-4851.

Casas-Fraile, L., Cornelis, F. M., Costamagna, D., Rico, A., Sampaolesi, M., de Munain, A. L., ... & Lories, R. J. (2018). Effect of frizzled related protein (FRZB) on muscles: an inverse relationship between FRZB and calpain-3 with potential impact on muscle dystrophy and osteoarthritis. Osteoarthritis and Cartilage, 26, S401.

Huang, X., Post, J. N., Zhong, L., Leijten, J., Larsson, S., Karperien, M., & Struglics, A. (2018). Dickkopf-related protein 1 and gremlin 1 show different response than frizzled-related protein in human synovial fluid following knee injury and in patients with osteoarthritis. Osteoarthritis and cartilage, 26(6), 834-843.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-FRZB Recombinant Antibody (4E5)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry