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Mouse Anti-FASN (AA 942-973) Recombinant Antibody (CBXF-0339) (CBMAB-F2213-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXF-0339
Antibody Isotype
IgM
Application
IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 942-973

Target

Full Name
Fatty Acid Synthase
Introduction
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Entrez Gene ID
2194
UniProt ID
P49327
Alternative Names
Fatty Acid Synthase; Short Chain Dehydrogenase/Reductase Family 27X, Member 1; FAS; EC 2.3.1.85; EC 6.3.3.1; EC 2.3.1; SDR27X1; OA-519;
Research Area
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Biological Process
Acetyl-CoA metabolic process Source: Ensembl
Cellular response to interleukin-4 Source: Ensembl
Establishment of endothelial intestinal barrier Source: Ensembl
Ether lipid biosynthetic process Source: Ensembl
Fatty acid biosynthetic process Source: UniProtKB-UniPathway
Fatty acid metabolic process Source: ProtInc
Glandular epithelial cell development Source: Ensembl
Mammary gland development Source: Ensembl
Monocyte differentiation Source: Ensembl
Neutrophil differentiation Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Cellular Location
Cytoplasm; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
PTM
S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1471 or Cys-2091 is important for the enzyme dimerization. In adipocytes, S-nitrosylation of Fatty acid synthase occurs under physiological conditions and gradually increases during adipogenesis.
More Infomation

Grunt, T. W., Lemberger, L., Colomer, R., López− Rodríguez, M. L., & Wagner, R. (2021). The pharmacological or genetic blockade of endogenous de novo fatty acid synthesis does not increase the uptake of exogenous lipids in ovarian cancer cells. Frontiers in oncology, 11, 610885.

Fhu, C. W., & Ali, A. (2020). Fatty acid synthase: an emerging target in cancer. Molecules, 25(17), 3935.

Che, L., Chi, W., Qiao, Y., Zhang, J., Song, X., Liu, Y., ... & Chen, L. (2020). Cholesterol biosynthesis supports the growth of hepatocarcinoma lesions depleted of fatty acid synthase in mice and humans. Gut, 69(1), 177-186.

Zhang, N., Zhao, H., & Zhang, L. (2019). Fatty acid synthase promotes the palmitoylation of chikungunya virus nsP1. Journal of virology, 93(3), e01747-18.

Bruning, U., Morales-Rodriguez, F., Kalucka, J., Goveia, J., Taverna, F., Queiroz, K. C., ... & Carmeliet, P. (2018). Impairment of angiogenesis by fatty acid synthase inhibition involves mTOR malonylation. Cell metabolism, 28(6), 866-880.

Jiang, L., Fang, X., Wang, H., Li, D., & Wang, X. (2018). Ovarian cancer-intrinsic fatty acid synthase prevents anti-tumor immunity by disrupting tumor-infiltrating dendritic cells. Frontiers in immunology, 9, 2927.

Zaytseva, Y. Y., Rychahou, P. G., Le, A. T., Scott, T. L., Flight, R. M., Kim, J. T., ... & Evers, M. (2018). Preclinical evaluation of novel fatty acid synthase inhibitors in primary colorectal cancer cells and a patient-derived xenograft model of colorectal cancer. Oncotarget, 9(37), 24787.

Jung, M. Y., Kang, J. H., Hernandez, D. M., Yin, X., Andrianifahanana, M., Wang, Y., ... & Leof, E. B. (2018). Fatty acid synthase is required for profibrotic TGF-β signaling. The FASEB Journal, 32(7), 3803.

Carroll, R. G., Zasłona, Z., Galván-Peña, S., Koppe, E. L., Sévin, D. C., Angiari, S., ... & O’Neill, L. A. (2018). An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activation. Journal of Biological Chemistry, 293(15), 5509-5521.

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For research use only. Not intended for any clinical use.

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