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Mouse Anti-EXOC4 Recombinant Antibody (14G1) (CBMAB-0279CQ)


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Summary

Host Animal
Mouse
Specificity
Rat, Mouse, Human
Clone
14G1
Antibody Isotype
IgG1
Application
IP, WB, FC, IHC-Fr

Basic Information

Immunogen
Fusion protein (Recombinant rat rSec8 (his-tagged)
Specificity
Rat, Mouse, Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
exocyst complex component 4
Introduction
The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. SEC8 is part of a multiprotein complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Hsu et al. (1996) purified rat brain Sec8, which was present in a 743-kD complex with Sec6 (608186) and 6 other proteins. The complex was broadly expressed in rat brain and displayed a plasma membrane localization in nerve terminals. Immunofluorescence microscopy detected Sec8 throughout cultured rat hippocampal neurons, including cell bodies, dendrites, axons, and nerve terminals. Brighter staining was observed at synapses. The Sec6/Sec8 complex coimmunoprecipitated with syntaxin (see 186590). Hsu et al. (1996) concluded that the rat Sec6/Sec8 complex is involved in neurotransmitter release via interactions with the core vesicle docking and fusion apparatus.
Entrez Gene ID
Human60412
Mouse20336
Rat116654
UniProt ID
HumanQ96A65
MouseO35382
RatQ62824
Alternative Names
SEC8; Sec8p; SEC8L1
Research Area
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Biological Process
Chemical synaptic transmission Source: GO_Central
Exocytosis Source: GO_Central
Golgi to plasma membrane transport Source: GO_Central
Paraxial mesoderm formation Source: Ensembl
Protein transport Source: UniProtKB-KW
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Vesicle docking involved in exocytosis Source: InterPro
Vesicle tethering involved in exocytosis Source: InterPro
Cellular Location
Midbody ring; Cell projection. Colocalizes with CNTRL/centriolin at the midbody ring (PubMed:16213214). Localizes at the leading edge of migrating cells (By similarity).
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For research use only. Not intended for any clinical use.

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