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Mouse Anti-EIF4EBP1 Recombinant Antibody (4B6G10) (CBMAB-1902CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4B6G10
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Purified recombinant fragment of 4E-BP1 expressed in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 4E Binding Protein 1
Introduction
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. Diseases associated with EIF4EBP1 include Tuberous Sclerosis and Tracheal Cancer. Among its related pathways are MET promotes cell motility and Translation Translation regulation by Alpha-1 adrenergic receptors.
Entrez Gene ID
1978
UniProt ID
Q13541
Alternative Names
Eukaryotic Translation Initiation Factor 4E Binding Protein 1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; EIF4E-Binding Protein 1; 4E-BP1; PHAS-I; 4EBP1; BP-1
Research Area
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Biological Process
Cellular response to hypoxia Source: Ensembl
G1/S transition of mitotic cell cycle Source: UniProtKB
Lung development Source: Ensembl
Negative regulation of protein-containing complex assembly Source: Ensembl
Negative regulation of translational initiation Source: UniProtKB
Positive regulation of mitotic cell cycle Source: UniProtKB
Response to amino acid starvation Source: Ensembl
Response to ethanol Source: Ensembl
Response to ischemia Source: Ensembl
TOR signaling Source: UniProtKB
Cellular Location
Cytosol; Postsynaptic cytosol; Nucleus; Cytoplasm; Glutamatergic synapse; Protein-containing complex
PTM
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.
Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
More Infomation

Hauffe, L., Picard, D., Musa, J., Remke, M., Grünewald, T. G., Rotblat, B., ... & Leprivier, G. (2022). Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) expression in glioblastoma is driven by ETS1-and MYBL2-dependent transcriptional activation. Cell death discovery, 8(1), 1-11.

Voeltzke, K., Scharov, K., Funk, C. M., Kahler, A., Picard, D., Hauffe, L., ... & Leprivier, G. (2022). EIF4EBP1 is transcriptionally upregulated by MYCN and associates with poor prognosis in neuroblastoma. Cell death discovery, 8(1), 1-12.

Zhang, L., & Su, X. (2022). Bioactive peptide inhibits acute myeloid leukemia cell proliferation by downregulating ALKBH5-mediated m6A demethylation of EIF4EBP1 and MLST8 mRNA. Cellular Oncology, 1-11.

Li, X., Zhang, Y., Su, L., Cai, L., Zhang, C., Zhang, J., ... & Huang, X. (2022). FGF21 alleviates pulmonary hypertension by inhibiting mTORC1/EIF4EBP1 pathway via H19. Journal of Cellular and Molecular Medicine, 26(10), 3005-3021.

Gui, C. P., Liao, B., Luo, C. G., Chen, Y. H., Tan, L., Tang, Y. M., ... & Wei, J. H. (2021). circCHST15 is a novel prognostic biomarker that promotes clear cell renal cell carcinoma cell proliferation and metastasis through the miR-125a-5p/EIF4EBP1 axis. Molecular Cancer, 20(1), 1-21.

Jiang, Y., Wu, S. Y., Chen, Y. L., Zhang, Z. M., Tao, Y. F., Xie, Y., ... & Lu, J. (2021). CEBPG promotes acute myeloid leukemia progression by enhancing EIF4EBP1. Cancer Cell International, 21(1), 1-12.

Yang, M., Yang, B., & Deng, D. (2021). Targeting of EIF4EBP1 by miR‐99a‐3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression. Journal of cellular and molecular medicine, 25(21), 10291-10305.

Wan, P., Chen, Z., Zhong, W., Jiang, H., Huang, Z., Peng, D., ... & Chen, N. (2020). BRDT is a novel regulator of eIF4EBP1 in renal cell carcinoma. Oncology Reports, 44(6), 2475-2486.

Akbarian, F., Tabatabaiefar, M. A., Shaygannejad, V., Shahpouri, M. M., Badihian, N., Sajjadi, R., ... & Noori-Daloii, M. R. (2020). Upregulation of MTOR, RPS6KB1, and EIF4EBP1 in the whole blood samples of Iranian patients with multiple sclerosis compared to healthy controls. Metabolic brain disease, 35(8), 1309-1316.

Li, S. Q., Feng, J., Yang, M., Ai, X. P., He, M., & Liu, F. (2020). Sauchinone: a prospective therapeutic agent-mediated EIF4EBP1 down-regulation suppresses proliferation, invasion and migration of lung adenocarcinoma cells. Journal of natural medicines, 74(4), 777-787.

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For research use only. Not intended for any clinical use.

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