Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-DLX5 Recombinant Antibody (2B6) (CBMAB-D1152-YC)


See all DLX5 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B6
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Immunogen
DLX5 (NP_005212, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. The immunogen sequence: MTGVFDRRVP SIRSGDFQAP FQTSAAMHHP SQESPTLPES SATDSDYYSP TGGAPHGYCS PTSASYGKAL NPYQYQYHGV NGSAGSYPAK AYADYSYASS YHQYGGAYNR *
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-110

Target

Full Name
distal-less homeobox 5
Introduction
DLX5 belongs to a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation.
Entrez Gene ID
1749
UniProt ID
P56178
Alternative Names
Distal-Less Homeobox 5; Distal-Less Homeo Box 5; Split Hand/Foot Malformation Type 1 With Sensorineural Hearing Loss; Homeobox Protein DLX-5; SHFM1D;
Function
Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.
Biological Process
Anatomical structure formation involved in morphogenesis Source: GO_Central
BMP signaling pathway Source: BHF-UCL
Cell differentiation Source: GO_Central
Cell population proliferation Source: UniProtKB
Cellular response to BMP stimulus Source: BHF-UCL
Embryonic limb morphogenesis Source: Ensembl
Endochondral ossification Source: UniProtKB
Epithelial cell differentiation Source: Ensembl
Face morphogenesis Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Interneuron axon guidance Source: Ensembl
Nervous system development Source: ProtInc
Olfactory bulb interneuron differentiation Source: Ensembl
Olfactory pit development Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: Ensembl
Positive regulation of epithelial cell proliferation Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Roof of mouth development Source: Ensembl
Skeletal system development Source: ProtInc
Cellular Location
Nucleus
Involvement in disease
Split-hand/foot malformation 1 with sensorineural hearing loss, autosomal recessive (SHFM1D):
A disease characterized by the association of split-hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting.
PTM
Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability (By similarity).
More Infomation

Mullen, R. D., Bellessort, B., Levi, G., & Behringer, R. D. (2022). Distal-less homeobox genes Dlx5/6 regulate Mulllerian duct regression. bioRxiv.

Kim, C. Y., Cho, D. H., Chung, D. J., Lee, S. H., Han, Y., & Lee, K. Y. (2021). Dlx5 Represses the Transcriptional Activity of PPARγ. Biological and Pharmaceutical Bulletin, 44(9), 1303-1308.

Zhang, G., Zhou, Y., Su, M., Yang, X., & Zeng, B. (2020). Inhibition of microRNA‐27b‐3p relieves osteoarthritis pain via regulation of KDM4B‐dependent DLX5. Biofactors, 46(5), 788-802.

Sun, S., Yang, F., Zhu, Y., & Zhang, S. (2020). KDM4A promotes the growth of non-small cell lung cancer by mediating the expression of Myc via DLX5 through the Wnt/β-catenin signaling pathway. Life Sciences, 262, 118508.

Yang, H., Fan, J., Cao, Y., Gao, R., & Fan, Z. (2019). Distal-less homeobox 5 promotes the osteo-/dentinogenic differentiation potential of stem cells from apical papilla by activating histone demethylase KDM4B through a positive feedback mechanism. Experimental Cell Research, 374(1), 221-230.

Yang, D., Okamura, H., & Qiu, L. (2018). Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα‐knockdown cells. Cell Biology International, 42(4), 403-410.

Lee, K. M., Park, K. H., Hwang, J. S., Lee, M., Yoon, D. S., Ryu, H. A., ... & Lee, J. W. (2018). Inhibition of STAT5A promotes osteogenesis by DLX5 regulation. Cell death & disease, 9(11), 1-13.

Lee, H. L., Qadir, A. S., Park, H. J., Chung, E., Lee, Y. S., Woo, K. M., ... & Baek, J. H. (2018). cAMP/protein kinase A signaling inhibits Dlx5 expression via activation of CREB and subsequent C/EBPβ induction in 3T3-L1 preadipocytes. International Journal of Molecular Sciences, 19(10), 3161.

Heo, J. S., Lee, S. G., & Kim, H. O. (2017). Distal-less homeobox 5 is a master regulator of the osteogenesis of human mesenchymal stem cells. International journal of molecular medicine, 40(5), 1486-1494.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DLX5 Recombinant Antibody (2B6)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry