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Mouse Anti-DGKA Recombinant Antibody (M5a) (CBMAB-D0780-YC)


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Summary

Host Animal
Mouse
Specificity
Rat
Clone
M5a
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
C-terminal portion (part of the catalytic domain) of rat DGKa
Specificity
Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
C-terminus

Target

Full Name
Diacylglycerol Kinase Alpha
Introduction
DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
Entrez Gene ID
140866
UniProt ID
P51556
Alternative Names
Diacylglycerol Kinase Alpha; Diacylglycerol Kinase, Alpha 80kDa; 80 KDa Diacylglycerol Kinase; Diglyceride Kinase Alpha; DAG Kinase Alpha; EC 2.7.1.107;
Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:2175712, PubMed:15544348).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:2175712, PubMed:15544348).

Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348).

Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129).
Biological Process
Diacylglycerol metabolic process Source: UniProtKB
Glycerolipid metabolic process Source: BHF-UCL
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Phosphatidic acid biosynthetic process Source: UniProtKB
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Cellular Location
Cytosol
More Infomation

Okada, N., Sugiyama, K., Shichi, S., Shirai, Y., Goto, K., Sakane, F., ... & Taketomi, A. (2022). Combination therapy for hepatocellular carcinoma with diacylglycerol kinase alpha inhibition and anti-programmed cell death-1 ligand blockade. Cancer Immunology, Immunotherapy, 71(4), 889-903.

Li, J., Shen, Y., Chen, Y., Zhang, Z., Ma, S., Wan, Q., ... & Yang, J. (2021). Structure of membrane diacylglycerol kinase in lipid bilayers. Communications biology, 4(1), 1-13.

Liu, C. S., Toth, R., Bakr, A., Goyal, A., Islam, M. S., Breuer, K., ... & Popanda, O. (2021). Epigenetic modulation of radiation-induced diacylglycerol kinase alpha expression prevents pro-fibrotic fibroblast response. Cancers, 13(10), 2455.

Liu, C. S., Schmezer, P., & Popanda, O. (2020). Diacylglycerol kinase alpha in radiation-induced fibrosis: Potential as a predictive marker or therapeutic target. Frontiers in Oncology, 10, 737.

Pan, Y., Deng, W., Xie, J., Zhang, S., Wan, E. C., Li, L., ... & Zhong, X. P. (2020). Graded diacylglycerol kinases α and ζ activities ensure mucosal‐associated invariant T‐cell development in mice. European journal of immunology, 50(2), 192-204.

Chen, J., Zhang, W., Wang, Y., Zhao, D., Wu, M., Fan, J., ... & Zhan, Q. (2019). The diacylglycerol kinase α (DGKα)/Akt/NF-κB feedforward loop promotes esophageal squamous cell carcinoma (ESCC) progression via FAK-dependent and FAK-independent manner. Oncogene, 38(14), 2533-2550.

Olmez, I., Love, S., Xiao, A., Manigat, L., Randolph, P., McKenna, B. D., ... & Purow, B. (2018). Targeting the mesenchymal subtype in glioblastoma and other cancers via inhibition of diacylglycerol kinase alpha. Neuro-oncology, 20(2), 192-202.

Kelly, E., Sharma, D., Wilkinson, C. J., & Williams, R. S. (2018). Diacylglycerol kinase (DGKA) regulates the effect of the epilepsy and bipolar disorder treatment valproic acid in Dictyostelium discoideum. Disease models & mechanisms, 11(9), dmm035600.

Valinciute, G., Weigel, C., Veldwijk, M. R., Oakes, C. C., Herskind, C., Wenz, F., ... & Popanda, O. (2017). BET-bromodomain inhibitors modulate epigenetic patterns at the diacylglycerol kinase alpha enhancer associated with radiation-induced fibrosis. Radiotherapy and Oncology, 125(1), 168-174.

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For research use only. Not intended for any clinical use.

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