Mouse Anti-CRYM Recombinant Antibody (CBFYM-1025) (V2LY-1206-LY1334)

Name: Mouse Anti-CRYM Recombinant Antibody (CBFYM-1025)
SKU: V2LY-1206-LY1334
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-1025

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 22-314 mapping at the C-terminus of human µ-crystallin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

crystallin, mu

Entrez Gene ID

1428

UniProt ID

Q14894

Function

Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.

Biological Process

Lysine catabolic process Source: Reactome
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Sensory perception of sound Source: UniProtKB
Thyroid hormone metabolic process Source: GO_Central
Thyroid hormone transport Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Deafness, autosomal dominant, 40 (DFNA40):
A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

More Infomation

References

Kinney, C. J., & Bloch, R. J. (2021). µ-Crystallin: A thyroid hormone binding protein. Endocrine Regulations, 55(2), 89-102.

Jank, B. J., Haas, M., Schnoell, J., Schlederer, M., Heiduschka, G., Kenner, L., & Kadletz-Wanke, L. (2021). µ-Crystallin Is Associated with Disease Outcome in Head and Neck Squamous Cell Carcinoma. Journal of Personalized Medicine, 11(12), 1330.

Kinney, C. J., O'Neill, A., Noland, K., Huang, W., Muriel, J., Lukyanenko, V., ... & Bloch, R. J. (2021). μ-Crystallin in mouse skeletal muscle promotes a shift from glycolytic toward oxidative metabolism. Current research in physiology, 4, 47-59.

Wang, M., Li, Q., Deng, A., Zhu, X., & Yang, J. (2020). Identification of a novel mutation in CRYM in a Chinese family with hearing loss using whole‑exome sequencing. Experimental and therapeutic medicine, 20(2), 1447-1454.

Ohkubo, Y., Sekido, T., Nishio, S. I., Sekido, K., Kitahara, J., Suzuki, S., & Komatsu, M. (2019). Loss of μ-crystallin causes PPARγ activation and obesity in high-fat diet-fed mice. Biochemical and biophysical research communications, 508(3), 914-920.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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