Mouse Anti-CRYBA1 Recombinant Antibody (CBCNC-376) (CBMAB-C2897-CN)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Cattle

Clone

CBCNC-376

Antibody Isotype

IgG1

Application

Basic Information

Specificity

Cattle

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2, 0.1 mM PMSF, 50% glycerol

Target

Full Name

Crystallin Beta A1

Introduction

CRYBA1 (Crystallin Beta A1) is a Protein Coding gene. Diseases associated with CRYBA1 include Cataract 10, Multiple Types and Cataract 25. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and structural constituent of eye lens. An important paralog of this gene is CRYBA4.

Entrez Gene ID

282202

UniProt ID

P11843

Alternative Names

Crystallin Beta A1; Eye Lens Structural Protein; CRYB1; Beta Crystallin A3 Chain Transcript LAM; Beta Crystallin A3 Chain Transcript CN; Beta Crystallin A3 Chain Transcript PS; Beta Crystallin A3 Chain Transcript TC; Truncated Beta Crystallin A3/A1 Chain;

Function

Crystallins are the dominant structural components of the vertebrate eye lens.

Biological Process

Lens development in camera-type eye Source: GO_Central
Negative regulation of cytokine production Source: Ensembl
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Negative regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Negative regulation of protein kinase B signaling Source: Ensembl
Negative regulation of TOR signaling Source: Ensembl
Positive regulation of anoikis Source: Ensembl
Visual perception Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm

Involvement in disease

Cataract 10, multiple types (CTRCT10):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. CTRCT10 includes congenital zonular with sutural opacities, among others. This is a form of zonular cataract with an erect Y-shaped anterior and an inverted Y-shaped posterior sutural opacities. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.

PTM

Specific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.

More Infomation

References

Ni, S. H., Zhang, J. M., & Zhao, J. (2022). A novel missense mutation of CRYBA1 in a northern Chinese family with inherited coronary cataract with blue punctate opacities. European Journal of Ophthalmology, 32(1), 193-199.

Xu, J., Wang, H., Wu, C., Wang, A., Wu, W., Xu, J., ... & Chen, X. (2021). Pathogenic mechanism of congenital cataract caused by the CRYBA1/A3-G91del variant and related intervention strategies. International Journal of Biological Macromolecules, 189, 44-52.

Shang, P., Stepicheva, N., Teel, K., Mccauley, A., Fitting, C. S., Hose, S., ... & Sinha, D. (2021). βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells. Communications biology, 4(1), 1-14.

Li, D., Jing, Q., & Jiang, Y. (2019). The identification and characterization of the p. G91 deletion in CRYBA1 in a Chinese family with congenital cataracts. BMC medical genetics, 20(1), 1-8.

Ghosh, S., Shang, P., Terasaki, H., Stepicheva, N., Hose, S., Yazdankhah, M., ... & Sinha, D. (2018). A role for βA3/A1-crystallin in type 2 EMT of RPE cells occurring in dry age-related macular degeneration. Investigative ophthalmology & visual science, 59(4), AMD104-AMD113.

Wang, K. J., Zha, X., Chen, D. D., & Zhu, S. Q. (2018). Mutation analysis of families with autosomal dominant congenital cataract: a recurrent mutation in the CRYBA1/A3 gene causing congenital nuclear cataract. Current Eye Research, 43(3), 304-307.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

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Rabbit Anti-CRYBA1 Recombinant Antibody (BA0376)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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