Rabbit Anti-CRABP1 Recombinant Antibody (D5W9A) (CBMAB-CP0366-LY)

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Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat, Chicken, Cattle

Clone

D5W9A

Antibody Isotype

IgG

Application

Basic Information

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CRABP1 protein.

Specificity

Human, Mouse, Rat, Chicken, Cattle

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cellular Retinoic Acid Binding Protein 1

Entrez Gene ID

Human	1381
Mouse	12903
Rat	25061
Cattle	282201
Chicken	374211

UniProt ID

Human	P29762
Mouse	P62965
Rat	P62966
Cattle	P62964
Chicken	P40220

Function

Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.

Biological Process

Multicellular organism development Source: ProtInc
Retinoic acid catabolic process Source: InterPro
Signal transduction Source: ProtInc

Cellular Location

Cytoplasm

More Infomation

References

Lin, Y. W., Nhieu, J., Wei, C. W., Lin, Y. L., Kagechika, H., & Wei, L. N. (2021). Regulation of exosome secretion by cellular retinoic acid binding protein 1 contributes to systemic anti-inflammation. Cell Communication and Signaling, 19(1), 1-11.

Enikeev, A. D., Komelkov, A. V., Axelrod, M. E., Galetsky, S. A., Kuzmichev, S. A., & Tchevkina, E. M. (2021). CRABP1 and CRABP2 protein levels correlate with each other but do not correlate with sensitivity of breast cancer cells to retinoic acid. Biochemistry (Moscow), 86(2), 217-229.

Lin, Y. L., Lin, Y. W., Nhieu, J., Zhang, X., & Wei, L. N. (2020). Sonic hedgehog-gli1 signaling and cellular retinoic acid binding protein 1 gene regulation in motor neuron differentiation and diseases. International journal of molecular sciences, 21(11), 4125.

Lin, Y. W., Park, S. W., Lin, Y. L., Burton, F. H., & Wei, L. N. (2020). Cellular retinoic acid binding protein 1 protects mice from high-fat diet-induced obesity by decreasing adipocyte hypertrophy. International journal of obesity, 44(2), 466-474.

Park, S. W., Nhieu, J., Persaud, S. D., Miller, M. C., Xia, Y., Lin, Y. W., ... & Wei, L. N. (2019). A new regulatory mechanism for Raf kinase activation, retinoic acid-bound Crabp1. Scientific reports, 9(1), 1-12.

Nagpal, I., & Wei, L. N. (2019). All-trans retinoic acid as a versatile cytosolic signal modulator mediated by CRABP1. International journal of molecular sciences, 20(15), 3610.

Park, S. W., Nhieu, J., Lin, Y. W., & Wei, L. N. (2019). All-trans retinoic acid attenuates isoproterenol-induced cardiac dysfunction through Crabp1 to dampen CaMKII activation. European journal of pharmacology, 858, 172485.

Lin, Y. L., Persaud, S. D., Nhieu, J., & Wei, L. N. (2017). Cellular retinoic acid–binding protein 1 modulates stem cell proliferation to affect learning and memory in male mice. Endocrinology, 158(9), 3004-3014.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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