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Mouse Anti-COX4I1 Recombinant Antibody (CBFYC-0437) (V2LY-1206-LY853)


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Published Data
V2LY-1206-LY853-1
Mouse Anti-COX4I1 Recombinant Antibody (CBFYC-0437) (V2LY-1206-LY853) in WB
Western blot analysis showed the expression of Ahi1 and GR in the mitochondria of hypothalamic tissues in control mice and Ahi1 KO mice. ...View More
Wang, B., Shi, H., Yang, B., Miao, Z., Sun, M., Yang, H., & Xu, X. (2023). The mitochondrial Ahi1/GR participates the regulation on mtDNA copy numbers and brain ATP levels and modulates depressive behaviors in mice. Cell Communication and Signaling, 21(1), 21.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-1206-LY853-2
Mouse Anti-COX4I1 Recombinant Antibody (CBFYC-0437) (V2LY-1206-LY853) in WB
PC12 cells were treated with Dex (20 μM) for 72 h. Ahi1 and GR were detected in the mitochondria by Western blotting. ...View More
Wang, B., Shi, H., Yang, B., Miao, Z., Sun, M., Yang, H., & Xu, X. (2023). The mitochondrial Ahi1/GR participates the regulation on mtDNA copy numbers and brain ATP levels and modulates depressive behaviors in mice. Cell Communication and Signaling, 21(1), 21.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-1206-LY853-3
Mouse Anti-COX4I1 Recombinant Antibody (CBFYC-0437) (V2LY-1206-LY853) in WB
Transcription regulators and protein contents involved in OxPhos pathway of MCF-7 MCTS. Histograms represent a double normalization against α-tubulin. Data shown represent the mean ± S.D. of at least three different preparations. ...View More
Pacheco-Velázquez, S. C., Ortega-Mejía, I. I., Vargas-Navarro, J. L., Padilla-Flores, J. A., Robledo-Cadena, D. X., Tapia-Martínez, G., ... & Rodríguez-Enríquez, S. (2022). 17-β Estradiol up-regulates energy metabolic pathways, cellular proliferation and tumor invasiveness in ER+ breast cancer spheroids. Frontiers in Oncology, 12, 1018137.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0437
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 86-169 mapping at the C-terminus of human COX4.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome C Oxidase Subunit 4I1
Entrez Gene ID
1327
UniProt ID
P13073
Alternative Names
Cytochrome C Oxidase Subunit 4I1; Cytochrome C Oxidase Subunit IV Isoform 1; Cytochrome C Oxidase Polypeptide IV; Cytochrome C Oxidase Subunit IV; COX IV-1; COX4;
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Biological Process
Generation of precursor metabolites and energy Source: ProtInc
Mitochondrial electron transport, cytochrome c to oxygen Source: GO_Central
Response to nutrient Source: Ensembl
Cellular Location
Mitochondrion inner membrane
Involvement in disease
Mitochondrial complex IV deficiency, nuclear type 16 (MC4DN16):
An autosomal recessive mitochondrial disorder with onset in infancy and variable manifestations. MC4DN16 features include feeding difficulties, poor overall growth, short stature, microcephaly, developmental regression, severe hypotonia, and seizures. Cerebral and cerebellar atrophy, and abnormal lesions in the basal ganglia can be observed on brain imaging. Patient tissues show decreased levels and activity of mitochondrial respiratory complex IV.
Topology
Mitochondrial matrix: 23-98
Helical: 99-124
Mitochondrial intermembrane: 125-169
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For research use only. Not intended for any clinical use.

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