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Mouse Anti-CLTC Recombinant Antibody (X22) (CBMAB-1394-CN)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Dog, Rat, Hamster, Cattle, Chicken
Clone
X22
Antibody Isotype
IgG1
Application
BL, ELISA, FC, IF, IHC, IHC-P, IM, IP, WB

Basic Information

Immunogen
Purified human brain clathrin heavy chain
Specificity
Human, Mouse, Dog, Rat, Hamster, Cattle, Chicken
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Concentration
6 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Clathrin Heavy Chain
Introduction
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. This protein plays a role in early autophagosome formation.
Entrez Gene ID
Human1213
Mouse67300
Rat54241
Hamster100756644
Cattle281080
Chicken395272
UniProt ID
HumanQ00610
MouseQ68FD5
RatP11442
HamsterA0A1U7QDR7
CattleP49951
ChickenF1NW23
Alternative Names
Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582).

The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825).

Plays a role in early autophagosome formation (PubMed:20639872).
Biological Process
Amyloid-beta clearance by transcytosis Source: ARUK-UCL
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Autophagy Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Clathrin coat assembly Source: CAFA
Clathrin-dependent endocytosis Source: ARUK-UCL
Intracellular protein transport Source: UniProtKB
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Mitotic cell cycle Source: UniProtKB
Negative regulation of hyaluronan biosynthetic process Source: UniProtKB
Negative regulation of protein localization to plasma membrane Source: UniProtKB
Osteoblast differentiation Source: UniProtKB
Post-Golgi vesicle-mediated transport Source: Reactome
Receptor internalization Source: BHF-UCL
Receptor-mediated endocytosis Source: UniProtKB
Regulation of mitotic spindle organization Source: UniProtKB
Retrograde transport, endosome to Golgi Source: UniProtKB
Transferrin transport Source: BHF-UCL
Wnt signaling pathway, planar cell polarity pathway Source: Reactome
Cellular Location
Spindle; Cytoplasmic vesicle membrane; Coated pit; Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.
Involvement in disease
Mental retardation, autosomal dominant 56 (MRD56):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
More Infomation

Itai, T., Miyatake, S., Tsuchida, N., Saida, K., Narahara, S., Tsuyusaki, Y., ... & Matsumoto, N. (2022). Novel CLTC variants cause new brain and kidney phenotypes. Journal of Human Genetics, 67(1), 1-7.

Bridge, J. A., Sumegi, J., Royce, T., Baker, M., & Linos, K. (2021). A novel CLTC‐FOSB gene fusion in pseudomyogenic hemangioendothelioma of bone. Genes, Chromosomes and Cancer, 60(1), 38-42.

Nabais Sá, M. J., Venselaar, H., Wiel, L., Trimouille, A., Lasseaux, E., Naudion, S., ... & Koolen, D. A. (2020). De novo CLTC variants are associated with a variable phenotype from mild to severe intellectual disability, microcephaly, hypoplasia of the corpus callosum, and epilepsy. Genetics in Medicine, 22(4), 797-802.

Manti, F., Nardecchia, F., Barresi, S., Venditti, M., Pizzi, S., Hamdan, F. F., ... & Leuzzi, V. (2019). Neurotransmitter trafficking defect in a patient with clathrin (CLTC) variation presenting with intellectual disability and early-onset parkinsonism. Parkinsonism & Related Disorders, 61, 207-210.

Anderson, R. H., Kerkvliet, J. G., Otta, J. J., Ross, A. D., Leiferman, P. C., Hoppe, A. D., & Francis, K. R. (2018). Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem cell research, 33, 95-99.

Latomanski, E. A., & Newton, H. J. (2018). Interaction between autophagic vesicles and the Coxiella-containing vacuole requires CLTC (clathrin heavy chain). Autophagy, 14(10), 1710-1725.

Lin, M. H., Liu, Y. C., Liu, S. Y., Chen, F. C., Yang, P. J., Li, G. H., ... & Yen, C. Y. (2018). Clathrin‐mediated endocytosis is required for ANE 30‐100K‐induced autophagy. Journal of Oral Pathology & Medicine, 47(1), 25-31.

Mizuta, H., Mushirobira, Y., Nagata, J., Todo, T., Hara, A., Reading, B. J., ... & Hiramatsu, N. (2017). Ovarian expression and localization of clathrin (Cltc) components in cutthroat trout, Oncorhynchus clarki: evidence for Cltc involvement in endocytosis of vitellogenin during oocyte growth. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 212, 24-34.

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For research use only. Not intended for any clinical use.

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