Mouse Anti-CLTA Recombinant Antibody (CBWJC-2823) (V2LY-1206-LY304)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBWJC-2823

Antibody Isotype

IgG1

Application

WB, IHC

Basic Information

Immunogen

Full length human recombinant protein of human CLTA produced in E. coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:2,000
IHC	1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

clathrin, light chain (Lca)

Entrez Gene ID

Human	1211
Mouse	12757
Rat	83800
Cattle	281078
Zebrafish	406318

UniProt ID

Human	P09496
Mouse	O08585
Rat	P08081
Cattle	P04973
Zebrafish	Q6P937

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Clathrin coat assembly Source: CAFA
Clathrin-dependent endocytosis Source: GO_Central
Intracellular protein transport Source: InterPro
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Post-Golgi vesicle-mediated transport Source: Reactome

Cellular Location

Spindle; Cytoplasmic vesicle membrane; Coated pit. Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

More Infomation

References

Redlingshöfer, L., McLeod, F., Chen, Y., Camus, M. D., Burden, J. J., Palomer, E., ... & Brodsky, F. M. (2020). Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo. Proceedings of the National Academy of Sciences, 117(38), 23527-23538.

Tsygankova, O. M., & Keen, J. H. (2019). A unique role for clathrin light chain A in cell spreading and migration. Journal of cell science, 132(10), jcs224030.

Biancospino, M., Buel, G. R., Niño, C. A., Maspero, E., Scotto di Perrotolo, R., Raimondi, A., ... & Polo, S. (2019). Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension. Nature communications, 10(1), 1-15.

Anderson, R. H., Kerkvliet, J. G., Otta, J. J., Ross, A. D., Leiferman, P. C., Hoppe, A. D., & Francis, K. R. (2018). Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem cell research, 33, 95-99.

Lu, Y., Wang, J., Guo, X., Yan, S., & Dai, J. (2017). Perfluorooctanoic acid affects endocytosis involving clathrin light chain A and microRNA-133b-3p in mouse testes. Toxicology and Applied Pharmacology, 318, 41-48.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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