Mouse Anti-CHN1 Recombinant Antibody (CBFYC-1843) (CBMAB-C1907-FY)

Name: Mouse Anti-CHN1 Recombinant Antibody (CBFYC-1843)
SKU: CBMAB-C1907-FY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBFYC-1843

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 144-183 mapping within an internal region of α-chimaerin of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Chimerin 1

Introduction

This gene encodes GTPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. Mutations in this gene are associated with Duane's retraction syndrome 2 (DURS2). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Entrez Gene ID

1123

UniProt ID

P15882

Alternative Names

Chimerin 1; Rho GTPase-Activating Protein 2; Chimerin (Chimaerin) 1; Alpha-Chimerin; Chimaerin 1; A-Chimaerin; N-Chimerin; ARHGAP2; CHN

Function

GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.

Biological Process

Ephrin receptor signaling pathway Source: UniProtKB
Intracellular signal transduction Source: InterPro
Motor neuron axon guidance Source: UniProtKB
Regulation of axonogenesis Source: UniProtKB
Regulation of GTPase activity Source: GO_Central
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Cytosol

Involvement in disease

Duane retraction syndrome 2 (DURS2): A form of Duane retraction syndrome, a congenital eye movement disorder characterized by a failure of cranial nerve VI (the abducens nerve) to develop normally, resulting in restriction or absence of abduction, adduction or both, narrowing of the palpebral fissure, and retraction of the globe on attempted adduction. Undiagnosed in children, it can lead to amblyopia, a permanent uncorrectable loss of vision.

PTM

Phosphorylated. Phosphorylation is EPHA4 kinase activity-dependent (By similarity).

More Infomation

References

Li, J. P., Zeng, S. H., Zhang, Y. H., & Liu, Y. J. (2021). Bioinformatics-based analysis of the association between the A1-chimaerin (CHN1) gene and gastric cancer. Bioengineered, 12(1), 2874-2889.

Zhao, H., Wang, L., Wang, S., Chen, X., Liang, M., Zhang, X., ... & Xu, X. (2021). CHN1 promotes epithelial–mesenchymal transition via the Akt/GSK-3β/Snail pathway in cervical carcinoma. Journal of Translational Medicine, 19(1), 1-12.

Zhou, T. C., Duan, W. H., Fu, X. L., Zhu, Q., Guo, L. Y., Zhou, Y., ... & Hu, M. (2020). Identification of a novel CHN1 p.(Phe213Val) variant in a large Han Chinese family with congenital Duane retraction syndrome. Scientific reports, 10(1), 1-9.

Liu, J., Li, Y., Chen, X., Xu, X., Zhao, H., Wang, S., ... & Wang, J. (2020). Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis. BMC cancer, 20(1), 1-13.

Biler, E. D., Ilim, O., Onay, H., & Uretmen, O. (2017). CHN1 gene mutation analysis in patients with Duane retraction syndrome. Journal of American Association for Pediatric Ophthalmology and Strabismus, 21(6), 472-475.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

CHN1 Matched Antibody Pair (270)

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CHN1 Matched Antibody Pair (270)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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