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Mouse Anti-CHIT1 Recombinant Antibody (CBFYC-1836) (CBMAB-C1900-FY)


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Published Data
CBMAB-C1900-FY-1
Mouse Anti-CHIT1 Recombinant Antibody (CBFYC-1836) (CBMAB-C1900-FY) in WB
Western blot imaging of CHIT1 protein expression. Both the 50 kDa and 39-42 kDa isoforms were significantly increased in BMDM from CHIT1-OE mice compared to controls (P = 0.0245 and 0.0168 respectively). ...View More
Yap, J., McCurdy, S., Alcala, M., Irei, J., Garo, J., Regan, W., ... & Boisvert, W. A. (2020). Expression of chitotriosidase in macrophages modulates atherosclerotic plaque formation in hyperlipidemic mice. Frontiers in Physiology, 11, 714.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1900-FY-2
Mouse Anti-CHIT1 Recombinant Antibody (CBFYC-1836) (CBMAB-C1900-FY) in IHC
Cryosections of the aortic sinus from both CHIT1-OE mice and littermate controls after 12 weeks of HFD were stained with biotinylated antibodies against CHIT1. ...View More
Yap, J., McCurdy, S., Alcala, M., Irei, J., Garo, J., Regan, W., ... & Boisvert, W. A. (2020). Expression of chitotriosidase in macrophages modulates atherosclerotic plaque formation in hyperlipidemic mice. Frontiers in Physiology, 11, 714.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1836
Antibody Isotype
IgM, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 150-215 mapping within an internal region of Chitotriosidase of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 22-137

Target

Full Name
Chitinase 1
Introduction
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
Entrez Gene ID
1118
UniProt ID
Q13231
Alternative Names
Chitinase 1; Chitinase 1 (Chitotriosidase); EC 3.2.1.14; Plasma Methylumbelliferyl Tetra-N-Acetylchitotetraoside Hydrolase; Chitotriosidase-1; Chitotriosidase
Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
Biological Process
Chitin catabolic process Source: UniProtKB
Immune response Source: UniProtKB
Neutrophil degranulation Source: Reactome
Polysaccharide catabolic process Source: UniProtKB-KW
Polysaccharide digestion Source: Reactome
Response to bacterium Source: ProtInc
Cellular Location
Secreted; Lysosome. A small proportion is lysosomal.
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For research use only. Not intended for any clinical use.

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