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Mouse Anti-CDX2 Recombinant Antibody (CBFYC-1662) (CBMAB-C1724-FY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1662
Antibody Isotype
IgG2b, κ
Application
WB, IF, ELISA, IP

Basic Information

Immunogen
Amino acids 261-292 near the C-terminus of Cdx2 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-314

Target

Full Name
Caudal Type Homeobox 2
Introduction
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.
Entrez Gene ID
1045
UniProt ID
Q99626
Alternative Names
Caudal Type Homeobox 2; Caudal-Type Homeobox Protein 2; CDX-3; CDX3; Caudal Type Homeo Box Transcription Factor 2; Caudal Type Homeobox Transcription Factor 2; Homeobox Protein MiniCDX2; Homeobox Protein CDX-2; CDX2/AS;
Function
Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity).
Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity).
Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity).
Binds to and activates transcription of LPH (By similarity).
Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity).
Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity).
Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).
Biological Process
Animal organ morphogenesis Source: GO_Central
Anterior/posterior axis specification Source: GO_Central
Blood vessel development Source: Ensembl
Cell differentiation Source: GO_Central
Endosome to lysosome transport Source: Ensembl
Establishment or maintenance of epithelial cell apical/basal polarity Source: Ensembl
Intestinal epithelial cell differentiation Source: UniProtKB
Labyrinthine layer development Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of cell differentiation Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: Ensembl
Regulation of somitogenesis Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Somatic stem cell population maintenance Source: Ensembl
Trophectodermal cell differentiation Source: Ensembl
Cellular Location
Nucleus
PTM
Ubiquitinated, leading to its degradation by the proteasome.
Phosphorylation at Ser-60 reduces transactivation capacity. Phosphorylation at Ser-283 reduces transactivation capacity and also increases ubiquitin-dependent proteasome degradation.
More Infomation

Stevens, S. J., Stumpel, C. T., Diderich, K. E., van Slegtenhorst, M. A., Abbott, M. A., Manning, C., ... & Brunner, H. G. (2021). The broader phenotypic spectrum of congenital caudal abnormalities associated with mutations in the caudal type homeobox 2 gene. Clinical Genetics.

Chen, T., Yang, C., Xi, Z., Chen, F., & Li, H. (2020). Reduced Caudal Type Homeobox 2 (CDX2) Promoter Methylation Is Associated with Curcumin’s Suppressive Effects on Epithelial-Mesenchymal Transition in Colorectal Cancer Cells. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 26, e926443-1.

Jiang, X., Li, S., Qiu, X., Cong, J., Zhou, J., & Miu, W. (2019). Curcumin inhibits cell viability and increases apoptosis of SW620 human colon adenocarcinoma cells via the caudal type homeobox-2 (CDX2)/Wnt/β-catenin pathway. Medical science monitor: international medical journal of experimental and clinical research, 25, 7451.

Vadakke-Madathil, S., LaRocca, G., Raedschelders, K., Yoon, J., Parker, S. J., Tripodi, J., ... & Chaudhry, H. W. (2019). Multipotent fetal-derived Cdx2 cells from placenta regenerate the heart. Proceedings of the National Academy of Sciences, 116(24), 11786-11795.

Zheng, J., He, S., Qi, J., Wang, X., Yu, J., Wu, Y., ... & Sun, X. (2017). Targeted CDX2 expression inhibits aggressive phenotypes of colon cancer cells in vitro and in vivo. International journal of oncology, 51(2), 478-488.

Pilati, C., Taieb, J., Balogoun, R., Marisa, L., De Reyniès, A., & Laurent-Puig, P. (2017). CDX2 prognostic value in stage II/III resected colon cancer is related to CMS classification. Annals of Oncology, 28(5), 1032-1035.

Zhang, B. Y., Jones, J. C., Briggler, A. M., Hubbard, J. M., Kipp, B. R., Sargent, D. J., ... & Grothey, A. (2017). Lack of caudal-type homeobox transcription factor 2 expression as a prognostic biomarker in metastatic colorectal cancer. Clinical colorectal cancer, 16(2), 124-128.

Saito, M., Okayama, H., Saito, K., Ando, J., Kumamoto, K., Nakamura, I., ... & Takenoshita, S. (2017). CDX2 is involved in microRNA‑associated inflammatory carcinogenesis in gastric cancer. Oncology letters, 14(5), 6184-6190.

Sun, X., Yang, Q., Rogers, C. J., Du, M., & Zhu, M. J. (2017). AMPK improves gut epithelial differentiation and barrier function via regulating Cdx2 expression. Cell Death & Differentiation, 24(5), 819-831.

Fan, H. B., Zhai, Z. Y., Li, X. G., Gao, C. Q., Yan, H. C., Chen, Z. S., & Wang, X. Q. (2017). CDX2 stimulates the proliferation of porcine intestinal epithelial cells by activating the mTORC1 and Wnt/β-catenin signaling pathways. International journal of molecular sciences, 18(11), 2447.

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For research use only. Not intended for any clinical use.

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