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Mouse Anti-CDK16 Recombinant Antibody (CBYY-C1756) (CBMAB-C3194-YY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C1756
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-70 mapping at the N-terminus of PCTAIRE-1 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin Dependent Kinase 16
Entrez Gene ID
5127
UniProt ID
Q00536
Alternative Names
Cyclin Dependent Kinase 7; Cyclin-Dependent Kinase 7 (MO15 Homolog, Xenopus Laevis, Cdk-Activating Kinase); TFIIH Basal Transcription Factor Complex Kinase Subunit; Cell Division Protein Kinase 7; CDK-Activating Kinase 1; 39 KDa Protein Kinase; EC 2.7.11.22; CDKN7; STK1; CAK1; MO15; CAK; Cyclin-Dependent Kinase 7 (Homolog Of Xenopus MO15 Cdk-Activating Kinase); Homolog Of Xenopus MO15 Cdk-Activating Kinase;
Function
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity).
Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Biological Process
Exocytosis Source: UniProtKB
Growth hormone secretion Source: UniProtKB
Neuron projection development Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: GO_Central
Spermatogenesis Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm; Secretory vesicle; Synaptosome. Colocalizes with insulin in pancreas islets. Recruited to the cell membrane by CCNY.
PTM
Phosphorylation at Ser-153 inhibits kinase activity.
More Infomation

Zhao, J. A., Nie, W., Dong, L., Liu, W., & Wei, W. (2021). A curcumin analogue GL63 inhibits the malignant behaviors of hepatocellular carcinoma by inactivating the JAK2/STAT3 signaling pathway via the circZNF83/miR‐324‐5p/CDK16 axis. Journal of Gastroenterology and Hepatology.

Dohmen, M., Krieg, S., Agalaridis, G., Zhu, X., Shehata, S. N., Pfeiffenberger, E., ... & Vervoorts, J. (2020). AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nature communications, 11(1), 1-18.

Vervoorts, J., Neumann, D., & Lüscher, B. (2020). The CCNY (cyclin Y)-CDK16 kinase complex: a new regulator of autophagy downstream of AMPK. Autophagy, 16(9), 1724-1726.

Si, Y., Liu, J., Shen, H., Zhang, C., Wu, Y., Huang, Y., ... & Liu, T. (2019). Fisetin decreases TET 1 activity and CCNY/CDK 16 promoter 5hmC levels to inhibit the proliferation and invasion of renal cancer stem cell. Journal of cellular and molecular medicine, 23(2), 1095-1105.

Shehata, S. N., Deak, M., Collodet, C., Spiegl, S. F., Geley, S., Sumpton, D., & Sakamoto, K. (2019). Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen. Cellular signalling, 59, 53-61.

Wang, H., Liu, H., Min, S., Shen, Y., Li, W., Chen, Y., & Wang, X. (2018). CDK16 overexpressed in non-small cell lung cancer and regulates cancer cell growth and apoptosis via a p27-dependent mechanism. Biomedicine & Pharmacotherapy, 103, 399-405.

Dixon-Clarke, S. E., Shehata, S. N., Krojer, T., Sharpe, T. D., von Delft, F., Sakamoto, K., & Bullock, A. N. (2017). Structure and inhibitor specificity of the PCTAIRE-family kinase CDK16. Biochemical Journal, 474(5), 699-713.

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For research use only. Not intended for any clinical use.

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