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Mouse Anti-CDK16 Recombinant Antibody (2E5) (CBMAB-A6482-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2E5
Antibody Isotype
IgG2b, κ
Application
WB, ELISA, IP, IF

Basic Information

Immunogen
PCTK1 (1 a.a. ~ 80 a.a) partial recombinant protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cyclin Dependent Kinase 16
Introduction
The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]
Entrez Gene ID
5127
UniProt ID
Q00536
Alternative Names
FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE
Function
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity).
Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Biological Process
Exocytosis Source: UniProtKB
Growth hormone secretion Source: UniProtKB
Neuron projection development Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: GO_Central
Spermatogenesis Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm; Secretory vesicle; Synaptosome. Colocalizes with insulin in pancreas islets. Recruited to the cell membrane by CCNY.
PTM
Phosphorylation at Ser-153 inhibits kinase activity.
More Infomation

Zhao, J. A., Nie, W., Dong, L., Liu, W., & Wei, W. (2021). A curcumin analogue GL63 inhibits the malignant behaviors of hepatocellular carcinoma by inactivating the JAK2/STAT3 signaling pathway via the circZNF83/miR‐324‐5p/CDK16 axis. Journal of Gastroenterology and Hepatology.

Dohmen, M., Krieg, S., Agalaridis, G., Zhu, X., Shehata, S. N., Pfeiffenberger, E., ... & Vervoorts, J. (2020). AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nature communications, 11(1), 1-18.

Vervoorts, J., Neumann, D., & Lüscher, B. (2020). The CCNY (cyclin Y)-CDK16 kinase complex: a new regulator of autophagy downstream of AMPK. Autophagy, 16(9), 1724-1726.

Si, Y., Liu, J., Shen, H., Zhang, C., Wu, Y., Huang, Y., ... & Liu, T. (2019). Fisetin decreases TET 1 activity and CCNY/CDK 16 promoter 5hmC levels to inhibit the proliferation and invasion of renal cancer stem cell. Journal of cellular and molecular medicine, 23(2), 1095-1105.

Shehata, S. N., Deak, M., Collodet, C., Spiegl, S. F., Geley, S., Sumpton, D., & Sakamoto, K. (2019). Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen. Cellular signalling, 59, 53-61.

Wang, H., Liu, H., Min, S., Shen, Y., Li, W., Chen, Y., & Wang, X. (2018). CDK16 overexpressed in non-small cell lung cancer and regulates cancer cell growth and apoptosis via a p27-dependent mechanism. Biomedicine & Pharmacotherapy, 103, 399-405.

Dixon-Clarke, S. E., Shehata, S. N., Krojer, T., Sharpe, T. D., von Delft, F., Sakamoto, K., & Bullock, A. N. (2017). Structure and inhibitor specificity of the PCTAIRE-family kinase CDK16. Biochemical Journal, 474(5), 699-713.

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For research use only. Not intended for any clinical use.

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