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Mouse Anti-CDC42BPB Recombinant Antibody (C5093) (CBMAB-C5093-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C5093
Antibody Isotype
IgG3, κ
Application
WB, IF, IP, ELISA

Basic Information

Immunogen
Amino acids 1637-1675 near the C-terminus of MRCKβ of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CDC42 binding protein kinase beta (DMPK-like)
Introduction
CDC42BPB (CDC42 Binding Protein Kinase Beta) is a Protein Coding gene. Diseases associated with CDC42BPB include Myotonic Dystrophy and Lung Large Cell Carcinoma Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDC42BPA.
Entrez Gene ID
9578
UniProt ID
Q9Y5S2
Alternative Names
CDC42 Binding Protein Kinase Beta; Myotonic Dystrophy Kinase-Related CDC42-Binding Kinase Beta; Myotonic Dystrophy Protein Kinase-Like Beta; DMPK-Like Beta; CDC42BP-Beta; EC 2.7.11.1; MRCK Beta; CDC42-Binding Protein Kinase Beta (DMPK-Like);
Function
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715, PubMed:21949762).
In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160).
Phosphorylates PPP1R12A (PubMed:21457715).
In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).
Biological Process
Actin cytoskeleton reorganization Source: UniProtKB
Actomyosin structure organization Source: UniProtKB
Cell migration Source: UniProtKB
Cytoskeleton organization Source: ProtInc
Establishment or maintenance of cell polarity Source: ProtInc
Intracellular signal transduction Source: InterPro
Peptidyl-threonine phosphorylation Source: GO_Central
Protein phosphorylation Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cytoplasm; Cell membrane; Cell junction; Lamellipodium. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity).
PTM
Proteolytically cleaved by caspases upon apoptosis induction.
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For research use only. Not intended for any clinical use.

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