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Mouse Anti-CDC42BPB Recombinant Antibody (C4931) (CBMAB-C4931-LY)


See all CDC42BPB antibodies
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Published Data
CBMAB-C4931-LY-1
Mouse Anti-CDC42BPB Recombinant Antibody (C4931) (CBMAB-C4931-LY) in Immunoblot
Immunoblot analysis of MRCKα and MRCKβ expression in wild-type and ko mice. ...View More
Kwa, M. Q., Brandao, R., Phung, T. H., Ge, J., Scieri, G., & Brakebusch, C. (2021). MRCKα is dispensable for breast cancer development in the MMTV-PyMT model. Cells, 10(4), 942.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C4931-LY-2
Mouse Anti-CDC42BPB Recombinant Antibody (C4931) (CBMAB-C4931-LY) in WB
Representative Western blotting for the indicated proteins of MSC with knockout of Rock1, Rock2, MRCKα, and MRCKβ. ...View More
Ge, J., Burnier, L., Adamopoulou, M., Kwa, M. Q., Schaks, M., Rottner, K., & Brakebusch, C. (2018). RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells. Journal of Biological Chemistry, 293(24), 9358-9369.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C4931
Antibody Isotype
IgG1, κ
Application
WB, IF, IP, ELISA

Basic Information

Immunogen
Amino acids 916-1040 mapping within an internal region of MRCKβ of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CDC42 binding protein kinase beta (DMPK-like)
Introduction
CDC42BPB (CDC42 Binding Protein Kinase Beta) is a Protein Coding gene. Diseases associated with CDC42BPB include Myotonic Dystrophy and Lung Large Cell Carcinoma Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDC42BPA.
Entrez Gene ID
9578
UniProt ID
Q9Y5S2
Alternative Names
CDC42 Binding Protein Kinase Beta; Myotonic Dystrophy Kinase-Related CDC42-Binding Kinase Beta; Myotonic Dystrophy Protein Kinase-Like Beta; DMPK-Like Beta; CDC42BP-Beta; EC 2.7.11.1; MRCK Beta; CDC42-Binding Protein Kinase Beta (DMPK-Like);
Function
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715, PubMed:21949762).
In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160).
Phosphorylates PPP1R12A (PubMed:21457715).
In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).
Biological Process
Actin cytoskeleton reorganization Source: UniProtKB
Actomyosin structure organization Source: UniProtKB
Cell migration Source: UniProtKB
Cytoskeleton organization Source: ProtInc
Establishment or maintenance of cell polarity Source: ProtInc
Intracellular signal transduction Source: InterPro
Peptidyl-threonine phosphorylation Source: GO_Central
Protein phosphorylation Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cytoplasm; Cell membrane; Cell junction; Lamellipodium. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity).
PTM
Proteolytically cleaved by caspases upon apoptosis induction.
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For research use only. Not intended for any clinical use.

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