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Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY)


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Published Data
CBMAB-C3052-YY-1
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in IHC
Infiltration of neutrophils (MPO+), T lymphocytes (CD3+), monocytes/MΦs (CD68+), M1 (B7-2+), and M2 (CD163+) MΦs by immunohistochemestry in the glomeruli of Ctrl, DN, and DN + MRS1754 rats. The images on the left correspond to the original capture, and the images on the right correspond to the deconvoluted color channel of the DAB stain obtained through ImageJ analysis. The images show the areas specific for glomeruli with the maginification of 400×. ...View More
Torres-Arévalo, Á., Nahuelpán, Y., Muñoz, K., Jara, C., Cappelli, C., Taracha-Wiśniewska, A., ... & Martín, R. S. (2023). A2BAR antagonism decreases the glomerular expression and secretion of chemoattractants for monocytes and the pro-fibrotic M2 macrophages polarization during diabetic nephropathy. International Journal of Molecular Sciences, 24(13), 10829.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3052-YY-2
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in IF
Bar = 200 μm Double immunofluorescence staining of Prx4 with M1 macrophage marker CD86, M2 macrophage marker CD163, and plasma cell marker CD138 in Wt tumors. Framed inserts indicate higher magnification. Bar = 100 μm. ...View More
Thapa, P., Jiang, H., Ding, N., Hao, Y., Alshahrani, A., Lee, E. Y., ... & Wei, Q. (2023). Loss of peroxiredoxin IV protects mice from azoxymethane/dextran sulfate sodium-induced colorectal cancer development. Antioxidants, 12(3), 677.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3052-YY-3
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in IHC
IHC of M1 macrophage marker CD86 showing lower staining in Prx4 KO tumor. ...View More
Thapa, P., Jiang, H., Ding, N., Hao, Y., Alshahrani, A., Lee, E. Y., ... & Wei, Q. (2023). Loss of peroxiredoxin IV protects mice from azoxymethane/dextran sulfate sodium-induced colorectal cancer development. Antioxidants, 12(3), 677.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3052-YY-4
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in IF
The IF staining images show the change of M1/M2 ratio after PAM treatment (scale bars: 5 μm). Data from five randomly selected field of view (FOV) were analyzed using the ImageJ software to determine the intensity of CD86 and CD206. The results are presented as mean ± SD (*p < 0.05, one-way ANOVA). ...View More
Wang, S., Wang, P., Thompson, R., Ostrikov, K., Xiao, Y., & Zhou, Y. (2023). Plasma-activated medium triggers immunomodulation and autophagic activity for periodontal regeneration. Bioengineering & Translational Medicine, 8(4), e10528.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3052-YY-5
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in WB
qPCR and WB analysis of the effect of adding PDTC (20 μM) on iNOS, CD86, IL-6, Arg1, CD206, and IL-10 expression in MH-S cells at 24 h. ...View More
Geng, C., Wang, X., Chen, J., Sun, N., Wang, Y., Li, Z., ... & Gong, Y. (2023). Repetitive Low-Level Blast Exposure via Akt/NF-κB Signaling Pathway Mediates the M1 Polarization of Mouse Alveolar Macrophage MH-S Cells. International Journal of Molecular Sciences, 24(13), 10596.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3052-YY-6
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in IF
Representative images of p-MLKL (green), CD86 (pink) and CD11b (red) staining. The nuclei were stained with DAPI (blue). Yellow arrows: p-MLKL+CD86+CD11b+ cells. Scale bar: 30 μm. ...View More
Yang, S., Chang, N., Li, W., Yang, T., Xue, R., Liu, J., ... & Li, L. (2023). Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis. Cell Death & Disease, 14(3), 175.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3052-YY-7
Mouse Anti-CD86 Recombinant Antibody (CBYY-C1614) (CBMAB-C3052-YY) in IHC
Anti-CD86 staining of tumor tissue from three genotypes and quantitative analysis of staining intensity. ...View More
Hao, Y., Jiang, H., Thapa, P., Ding, N., Alshahrani, A., Fujii, J., ... & Wei, Q. (2023). Critical role of the Sulfiredoxin-Peroxiredoxin IV Axis in urethane-induced non-small cell lung cancer. Antioxidants, 12(2), 367.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C1614
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 24-223 of B7-2 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD86 Molecule
Entrez Gene ID
942
UniProt ID
P42081
Alternative Names
CD86 Molecule; CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen); CTLA-4 Counter-Receptor B7.2; CD28LG2; FUN-1; BU63; B70; B-Lymphocyte Activation Antigen B7-2;
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824).
Also involved in the regulation of B cells function, plays a role in regulating the level of IgG1 produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.
Biological Process
Activation of phospholipase C activity Source: UniProtKB
Activation of protein kinase C activity Source: UniProtKB
Adaptive immune response Source: UniProtKB-KW
B cell activation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Immune response Source: GO_Central
Negative regulation of T cell proliferation Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of immunoglobulin production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of lymphotoxin A production Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of T-helper 2 cell differentiation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
T cell costimulation Source: GO_Central
Cellular Location
Cell membrane
Topology
Extracellular: 24-247
Helical: 248-268
Cytoplasmic: 269-329
PTM
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
More Infomation

Watanabe, A., Inoue, N., Watanabe, M., Yamamoto, M., Ozaki, H., Hidaka, Y., & Iwatani, Y. (2020). Increases of CD80 and CD86 expression on peripheral blood cells and their gene polymorphisms in autoimmune thyroid disease. Immunological investigations, 49(1-2), 191-203.

Tanaka, S., Ise, W., Inoue, T., Ito, A., Ono, C., Shima, Y., ... & Kurosaki, T. (2020). Tet2 and Tet3 in B cells are required to repress CD86 and prevent autoimmunity. Nature immunology, 21(8), 950-961.

de Vos, L., Grünwald, I., Bawden, E. G., Dietrich, J., Scheckenbach, K., Wiek, C., ... & Dietrich, D. (2020). The landscape of CD28, CD80, CD86, CTLA4, and ICOS DNA methylation in head and neck squamous cell carcinomas. Epigenetics, 15(11), 1195-1212.

Haabeth, O. A. W., Blake, T. R., McKinlay, C. J., Tveita, A. A., Sallets, A., Waymouth, R. M., ... & Levy, R. (2019). Local delivery of Ox40l, Cd80, and Cd86 mRNA kindles global anticancer immunity. Cancer research, 79(7), 1624-1634.

Nombela, I., Requena-Platek, R., Morales-Lange, B., Chico, V., Puente-Marin, S., Ciordia, S., ... & Ortega-Villaizan, M. D. M. (2019). Rainbow trout red blood cells exposed to viral hemorrhagic septicemia virus up-regulate antigen-processing mechanisms and MHC I&II, CD86, and CD83 antigen-presenting cell markers. Cells, 8(5), 386.

Kleinpeter, P., Remy-Ziller, C., Winter, E., Gantzer, M., Nourtier, V., Kempf, J., ... & Marchand, J. B. (2019). By binding CD80 and CD86, the vaccinia virus M2 protein blocks their interactions with both CD28 and CTLA4 and potentiates CD80 binding to PD-L1. Journal of virology, 93(11), e00207-19.

Xiong, A., Wang, J., Mao, X. L., Jiang, Y., & Fan, Y. (2019). MiR‐199a‐3p modulates the function of dendritic cells involved in transplantation tolerance by targeting CD86. Hla, 94(6), 493-503.

Pinto, B. F., Medeiros, N. I., Teixeira-Carvalho, A., Eloi-Santos, S. M., Fontes-Cal, T., Rocha, D. A., ... & Gomes, J. A. (2018). CD86 expression by monocytes influences an immunomodulatory profile in asymptomatic patients with chronic Chagas disease. Frontiers in immunology, 9, 454.

Han, L., Shen, L., Zhu, Y., & Qiu, Y. (2017). A monoclonal antibody against CD86 and its protection in a murine lupus nephritis model of chronic graft-versus-host disease. Immunopharmacology and immunotoxicology, 39(5), 285-291.

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For research use only. Not intended for any clinical use.

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