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Rat Anti-CD86 Recombinant Antibody (CBXC-1637) (CBMAB-C2921-CQ)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBXC-1637
Antibody Isotype
IgG2a
Application
FC, IF, IP, WB

Basic Information

Immunogen
LPS activated B cells of mouse.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD86 Molecule
Introduction
CD86 (CD86 Molecule) is a Protein Coding gene. Diseases associated with CD86 include Gallbladder Squamous Cell Carcinoma and Myocarditis. Among its related pathways are Cytokine Signaling in Immune system and Th2 Differentiation Pathway. Gene Ontology (GO) annotations related to this gene include receptor binding and coreceptor activity. An important paralog of this gene is CD80.
Entrez Gene ID
12524
UniProt ID
P42082
Alternative Names
CD86 Molecule; CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen); CTLA-4 Counter-Receptor B7.2; CD28LG2; FUN-1; BU63; B70; B-Lymphocyte Activation Antigen B7-2;
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824).
Also involved in the regulation of B cells function, plays a role in regulating the level of IgG1 produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.
Biological Process
Activation of phospholipase C activity Source: UniProtKB
Activation of protein kinase C activity Source: UniProtKB
Adaptive immune response Source: UniProtKB-KW
B cell activation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Immune response Source: GO_Central
Negative regulation of T cell proliferation Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of immunoglobulin production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of lymphotoxin A production Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of T-helper 2 cell differentiation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
T cell costimulation Source: GO_Central
Cellular Location
Cell membrane
Topology
Extracellular: 24-247
Helical: 248-268
Cytoplasmic: 269-329
PTM
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
More Infomation

Watanabe, A., Inoue, N., Watanabe, M., Yamamoto, M., Ozaki, H., Hidaka, Y., & Iwatani, Y. (2020). Increases of CD80 and CD86 expression on peripheral blood cells and their gene polymorphisms in autoimmune thyroid disease. Immunological investigations, 49(1-2), 191-203.

Tanaka, S., Ise, W., Inoue, T., Ito, A., Ono, C., Shima, Y., ... & Kurosaki, T. (2020). Tet2 and Tet3 in B cells are required to repress CD86 and prevent autoimmunity. Nature immunology, 21(8), 950-961.

de Vos, L., Grünwald, I., Bawden, E. G., Dietrich, J., Scheckenbach, K., Wiek, C., ... & Dietrich, D. (2020). The landscape of CD28, CD80, CD86, CTLA4, and ICOS DNA methylation in head and neck squamous cell carcinomas. Epigenetics, 15(11), 1195-1212.

Haabeth, O. A. W., Blake, T. R., McKinlay, C. J., Tveita, A. A., Sallets, A., Waymouth, R. M., ... & Levy, R. (2019). Local delivery of Ox40l, Cd80, and Cd86 mRNA kindles global anticancer immunity. Cancer research, 79(7), 1624-1634.

Nombela, I., Requena-Platek, R., Morales-Lange, B., Chico, V., Puente-Marin, S., Ciordia, S., ... & Ortega-Villaizan, M. D. M. (2019). Rainbow trout red blood cells exposed to viral hemorrhagic septicemia virus up-regulate antigen-processing mechanisms and MHC I&II, CD86, and CD83 antigen-presenting cell markers. Cells, 8(5), 386.

Kleinpeter, P., Remy-Ziller, C., Winter, E., Gantzer, M., Nourtier, V., Kempf, J., ... & Marchand, J. B. (2019). By binding CD80 and CD86, the vaccinia virus M2 protein blocks their interactions with both CD28 and CTLA4 and potentiates CD80 binding to PD-L1. Journal of virology, 93(11), e00207-19.

Xiong, A., Wang, J., Mao, X. L., Jiang, Y., & Fan, Y. (2019). MiR‐199a‐3p modulates the function of dendritic cells involved in transplantation tolerance by targeting CD86. Hla, 94(6), 493-503.

Pinto, B. F., Medeiros, N. I., Teixeira-Carvalho, A., Eloi-Santos, S. M., Fontes-Cal, T., Rocha, D. A., ... & Gomes, J. A. (2018). CD86 expression by monocytes influences an immunomodulatory profile in asymptomatic patients with chronic Chagas disease. Frontiers in immunology, 9, 454.

Han, L., Shen, L., Zhu, Y., & Qiu, Y. (2017). A monoclonal antibody against CD86 and its protection in a murine lupus nephritis model of chronic graft-versus-host disease. Immunopharmacology and immunotoxicology, 39(5), 285-291.

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For research use only. Not intended for any clinical use.

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