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Mouse Anti-CD80 Recombinant Antibody (CBYY-0978) (CBMAB-0981-YY)


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Published Data
CBMAB-0981-YY-1
Mouse Anti-CD80 Recombinant Antibody (CBYY-0978) (CBMAB-0981-YY) in IHC
Representative images of the staining for CD80 in the lung sections of wild type and langerin-KO mouse after administration of L4 and elastase. Arrowheads indicate CD80-positive cells. The number of CD80-positive cells were counted in random 20 fields in each mouse. ∗∗∗∗p < 0.0001. Bars indicate 50 μm. ...View More
Ohkawa, Y., Kanto, N., Nakano, M., Fujinawa, R., Kizuka, Y., Johnson, E. L., ... & Taniguchi, N. (2023). Involvement of langerin in the protective function of a keratan sulfate-based disaccharide in an emphysema mouse model. Journal of Biological Chemistry, 299(8).
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-0981-YY-2
Mouse Anti-CD80 Recombinant Antibody (CBYY-0978) (CBMAB-0981-YY) in WB
Western blot and densitometry evaluations of CTLA-4, B7-1 and B7-2 accumulation in lungs from WT mice treated with vehicle (B16-) or B16 cells (B16 +). ...View More
Ma, B., Kamle, S., Akosman, B., Khan, H., Lee, C. M., Lee, C. G., & Elias, J. A. (2022). CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis. Frontiers in immunology, 13, 1056397.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-0981-YY-3
Mouse Anti-CD80 Recombinant Antibody (CBYY-0978) (CBMAB-0981-YY) in WB
Representative immunoblots depicting the inhibitory effects of GSK2879552 treatment on the examined protein expression levels in the aorta of ApoE-/- (HD) mice. ...View More
Manea, S. A., Vlad, M. L., Lazar, A. G., Muresian, H., Simionescu, M., & Manea, A. (2022). Pharmacological inhibition of lysine-specific demethylase 1A reduces atherosclerotic lesion formation in Apolipoprotein E-deficient mice by a mechanism involving decreased oxidative stress and inflammation; potential implications in human atherosclerosis. Antioxidants, 11(12), 2382.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0978
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Amino acids 35-242 of B7-1 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD80 Molecule
Introduction
CD80 (CD80 Molecule) is a Protein Coding gene.
Diseases associated with CD80 include Focal Segmental Glomerulosclerosis and Vaccinia.
Among its related pathways are PEDF Induced Signaling and Cytokine Signaling in Immune system.
Gene Ontology (GO) annotations related to this gene include coreceptor activity.
An important paralog of this gene is CD86.
Entrez Gene ID
941
UniProt ID
P33681
Alternative Names
CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1;
Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.
Biological Process
Cell surface receptor signaling pathway Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Immune response Source: GO_Central
Intracellular signal transduction Source: UniProtKB
Negative regulation of T cell proliferation Source: GO_Central
Positive regulation of granulocyte macrophage colony-stimulating factor production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of signal transduction Source: UniProtKB
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of T-helper 1 cell differentiation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
T cell costimulation Source: GO_Central
Cellular Location
Membrane
Topology
Extracellular: 35-242
Helical: 243-263
Cytoplasmic: 264-288
More Infomation

Tekguc, M., Wing, J. B., Osaki, M., Long, J., & Sakaguchi, S. (2021). Treg-expressed CTLA-4 depletes CD80/CD86 by trogocytosis, releasing free PD-L1 on antigen-presenting cells. Proceedings of the National Academy of Sciences, 118(30).

Zhao, Y., Lee, C. K., Lin, C. H., Gassen, R. B., Xu, X., Huang, Z., ... & Hui, E. (2019). PD-L1: CD80 cis-heterodimer triggers the co-stimulatory receptor CD28 while repressing the inhibitory PD-1 and CTLA-4 pathways. Immunity, 51(6), 1059-1073.

Haabeth, O. A. W., Blake, T. R., McKinlay, C. J., Tveita, A. A., Sallets, A., Waymouth, R. M., ... & Levy, R. (2019). Local delivery of Ox40l, Cd80, and Cd86 mRNA kindles global anticancer immunity. Cancer research, 79(7), 1624-1634.

Jiménez-Uribe, A. P., Valencia-Martínez, H., Carballo-Uicab, G., Vallejo-Castillo, L., Medina-Rivero, E., Chacón-Salinas, R., ... & Pérez-Tapia, S. M. (2019). CD80 expression correlates with IL-6 production in THP-1-like macrophages costimulated with LPS and dialyzable leukocyte extract (Transferon®). Journal of immunology research, 2019.

Kleinpeter, P., Remy-Ziller, C., Winter, E., Gantzer, M., Nourtier, V., Kempf, J., ... & Marchand, J. B. (2019). By binding CD80 and CD86, the vaccinia virus M2 protein blocks their interactions with both CD28 and CTLA4 and potentiates CD80 binding to PD-L1. Journal of virology, 93(11), e00207-19.

Li, W., Kim, T. I., Kim, J. H., & Chung, H. S. (2019). Immune checkpoint PD-1/PD-L1 CTLA-4/CD80 are blocked by Rhus verniciflua Stokes and its active compounds. Molecules, 24(22), 4062.

Huang, Y., Wang, Z., Zheng, Q., Tang, J., Cai, J., Lu, Y., & Jian, J. (2018). Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 72, 95-103.

Siokis, A., Robert, P. A., Demetriou, P., Dustin, M. L., & Meyer-Hermann, M. (2018). F-actin-driven CD28-CD80 localization in the immune synapse. Cell reports, 24(5), 1151-1162.

Bengsch, F., Knoblock, D. M., Liu, A., McAllister, F., & Beatty, G. L. (2017). CTLA-4/CD80 pathway regulates T cell infiltration into pancreatic cancer. Cancer Immunology, Immunotherapy, 66(12), 1609-1617.

Mo, Z. Q., Wang, J. L., Yang, M., Ni, L. Y., Wang, H. Q., Lao, G. F., ... & Dan, X. M. (2017). Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 post Cryptocaryon irritans infection. Fish & shellfish immunology, 67, 467-474.

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For research use only. Not intended for any clinical use.

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