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Rat Anti-CD63 Recombinant Antibody (7G4.2E8) (CBMAB-C8725-LY)


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Published Data
CBMAB-C8725-LY-1
Rat Anti-CD63 Recombinant Antibody (7G4.2E8) (CBMAB-C8725-LY) in WB
Western blot analysis of isolated exosomes. The presence of canonical exosome proteins and the HSP70 decreasing indicate a pure exosome preparation. ...View More
Chen, Y. Y., Lee, Y. H., Wang, B. J., Chen, R. J., & Wang, Y. J. (2022). Skin damage induced by zinc oxide nanoparticles combined with UVB is mediated by activating cell pyroptosis via the NLRP3 inflammasome-autophagy-exosomal pathway. Particle and fibre toxicology, 19, 1-22. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Rat
Specificity
Mouse, Human
Clone
7G4.2E8
Antibody Isotype
IgG2a, λ
Application
WB, FC

Basic Information

Immunogen
Partial recombinant mouse CD63 protein (between amino acids 100-250).
Host Species
Rat
Specificity
Mouse, Human
Antibody Isotype
IgG2a, λ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC2 μg/10^6 cells
WB2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD63 Molecule
Introduction
CD63 (CD63 Molecule) is a Protein Coding gene. Diseases associated with CD63 include Hermansky-Pudlak Syndrome and Melanoma. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System.

An important paralog of this gene is TSPAN33.
Entrez Gene ID
Human967
Mouse12512
UniProt ID
HumanP08962
MouseP41731
Function
Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Biological Process
Cell-matrix adhesion Source: UniProtKB
Cell migration Source: UniProtKB
Cellular protein localization Source: MGI
Endosome to melanosome transport Source: UniProtKB
Neutrophil degranulation Source: Reactome
Pigment granule maturation Source: UniProtKB
Platelet degranulation Source: Reactome
Positive regulation of integrin-mediated signaling pathway Source: UniProtKB
Positive regulation of receptor internalization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of potassium ion transmembrane transport Source: MGI
Regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB
Cellular Location
Lysosome membrane; Cell membrane; Extracellular exosome; Late endosome membrane; Multivesicular body; Melanosome; Cell surface. Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903).
Topology
Cytoplasmic: 2-11
Helical: 12-32
Extracellular: 33-51
Helical: 52-72
Cytoplasmic: 73-81
Helical: 82-102
Extracellular: 103-203
Helical: 204-224
Cytoplasmic: 225-238
PTM
Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
More Infomation

Fan, Z. H., Xu, Y., Luo, W., He, X. C., Zheng, T. T., Zhang, J. J., ... & Lee, X. Z. (2021). Molecular cloning and characterization of CD63 in common carp infected with koi herpesvirus. Developmental & Comparative Immunology, 121, 104102.

Mathieu, M., Névo, N., Jouve, M., Valenzuela, J. I., Maurin, M., Verweij, F. J., ... & Théry, C. (2021). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nature Communications, 12(1), 1-18.

Mathieu, M., Névo, N., Jouve, M., Valenzuela, J. I., Maurin, M., Verweij, F., ... & Théry, C. (2020). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking and synchronized extracellular vesicle release of CD9 and CD63. bioRxiv.

Dogrammatzis, C., Deschamps, T., & Kalamvoki, M. (2019). Biogenesis of extracellular vesicles during herpes simplex virus 1 infection: role of the CD63 tetraspanin. Journal of virology, 93(2), e01850-18.

Wang, L., Giri, B. R., Chen, Y., Xia, T., Liu, J., Li, H., ... & Cheng, G. (2018). Molecular characterization, expression profile, and preliminary evaluation of diagnostic potential of CD63 in Schistosoma japonicum. Parasitology research, 117(11), 3625-3631.

Hurwitz, S. N., Cheerathodi, M. R., Nkosi, D., York, S. B., & Meckes Jr, D. G. (2018). Tetraspanin CD63 bridges autophagic and endosomal processes to regulate exosomal secretion and intracellular signaling of Epstein-Barr virus LMP1. Journal of virology, 92(5), e01969-17.

Priyathilaka, T. T., Bathige, S. D. N. K., Herath, H. M. L. P. B., Lee, S., & Lee, J. (2017). Molecular identification of disk abalone (Haliotis discus discus) tetraspanin 33 and CD63: Insights into potent players in the disk abalone host defense system. Fish & shellfish immunology, 69, 173-184.

Raaben, M., Jae, L. T., Herbert, A. S., Kuehne, A. I., Stubbs, S. H., Chou, Y. Y., ... & Whelan, S. P. (2017). NRP2 and CD63 are host factors for Lujo virus cell entry. Cell host & microbe, 22(5), 688-696.

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For research use only. Not intended for any clinical use.

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