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Mouse Anti-CD177 Recombinant Antibody (CBYY-C0159) (CBMAB-C0507-YY)


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Published Data
CBMAB-C0507-YY-1
Mouse Anti-CD177 Recombinant Antibody (CBYY-C0159) (CBMAB-C0507-YY) in IHC
Immunohistochemistry for CD177 antigen detection in LV confirms transcriptomic profiling at the protein level. Staining was observed throughout the myocardium and was upregulated by PD in LV tissue from both Sham and 5/6Nx. Positive CD177 antigen signal was enriched within cardiomyocyte nuclei in Control animals. PD increases nuclear and cytoplasmic expression in LV tissue from Sham rats, while primarily increasing cytoplasmic staining in LV tissue from 5/6Nx rats. Cal. bar = 100 mm. ...View More
Nasci, V. L., Liu, P., Marks, A. M., Williams, A. C., & Kriegel, A. J. (2023). Transcriptomic analysis identifies novel candidates in cardiorenal pathology mediated by chronic peritoneal dialysis. Scientific Reports, 13(1), 10051. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0507-YY-2
Mouse Anti-CD177 Recombinant Antibody (CBYY-C0159) (CBMAB-C0507-YY) in WB
Western blot analyses of HNA-2 protein expression in whole blood leukocytes using alloantibodies and the mAb targeting N-terminus of HNA-2. ...View More
Li, Y., Mair, D. C., Schuller, R. M., Li, L., & Wu, J. (2015). Genetic mechanism of human neutrophil antigen 2 deficiency and expression variations. PLoS genetics, 11(5), e1005255. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C0159
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB, IHC-P

Basic Information

Immunogen
Amino acids 27-247 mapping within an internal region of CD177 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD177 Molecule
Entrez Gene ID
Human57126
Monkey103234781
UniProt ID
HumanQ8N6Q3
MonkeyA0A2K5VCQ1
Function
In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407).
In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308).
However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681).
Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208).
By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).
Biological Process
Blood coagulation Source: Reactome
Cell adhesion Source: UniProtKB
Cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
Cell-cell junction maintenance Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Leukocyte cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Neutrophil degranulation Source: Reactome
Neutrophil extravasation Source: UniProtKB
Neutrophil migration Source: UniProtKB
Positive regulation of neutrophil degranulation Source: UniProtKB
Positive regulation of superoxide anion generation Source: UniProtKB
Protein localization to cell surface Source: UniProtKB
Regulation of endocytosis Source: UniProtKB
Regulation of integrin-mediated signaling pathway Source: UniProtKB
Cellular Location
Cell membrane; Secreted; Membrane raft; Cytoplasmic granule membrane; Lamellipodium. Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).
PTM
N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
More Infomation

Browne, T., Wroe, E., Keen, L., & Poles, A. (2021). Human neutrophil antigen 2 sequence‐based typing: Joining the hunt for the CD177 answer. Vox Sanguinis.

Saha, R., Pradhan, S. S., Das, P., Mishra, P., Singh, R., Sivaramakrishnan, V., & Acharya, P. (2021). Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports, 11(1), 1-16.

Dahlstrand Rudin, A., Amirbeagi, F., Davidsson, L., Khamzeh, A., Thorbert Mros, S., Thulin, P., ... & Bylund, J. (2021). The neutrophil subset defined by CD177 expression is preferentially recruited to gingival crevicular fluid in periodontitis. Journal of Leukocyte Biology, 109(2), 349-362.

Lévy, Y., Wiedemann, A., Hejblum, B. P., Durand, M., Lefebvre, C., Surénaud, M., ... & French COVID cohort study group. (2021). CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. Iscience, 24(7), 102711.

Kim, M. C., Borcherding, N., Ahmed, K. K., Voigt, A. P., Vishwakarma, A., Kolb, R., ... & Zhang, W. (2021). CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nature communications, 12(1), 1-13.

Huang, Y. (2019). 4311 Decreased CD177 expression and its association with intravenous immunoglobulin resistance in Kawasaki disease. European Heart Journal, 40(Supplement_1), ehz745-0156.

Deng, H., Hu, N., Wang, C., Chen, M., & Zhao, M. H. (2018). Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA. Arthritis research & therapy, 20(1), 1-10.

Bai, M., Grieshaber-Bouyer, R., Wang, J., Schmider, A. B., Wilson, Z. S., Zeng, L., ... & Nigrovic, P. A. (2017). CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood, The Journal of the American Society of Hematology, 130(19), 2092-2100.

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For research use only. Not intended for any clinical use.

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