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Rabbit Anti-CBX3 Monoclonal Antibody (CBFYH-2922) (CBMAB-H0859-FY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBFYH-2922
Antibody Isotype
IgG
Application
WB, IHC-P, ELISA

Basic Information

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HP1 gamma/CBX3.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:1,000
IHC-P1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 50% glycerol, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Chromobox 3
Introduction
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Entrez Gene ID
Human11335
Mouse12417
UniProt ID
HumanQ13185
MouseP23198
Alternative Names
Chromobox 3
Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
Biological Process
Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin remodeling Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of transcription, DNA-templated Source: UniProtKB
Rhythmic process Source: UniProtKB-KW
Cellular Location
Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).
PTM
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
More Infomation

Huang, Y., Lin, Y., Song, X., & Wu, D. (2021). LINC00857 contributes to proliferation and lymphomagenesis by regulating miR-370-3p/CBX3 axis in diffuse large B-cell lymphoma. Carcinogenesis, 42(5), 733-741.

Niu, H., Chen, P., Fan, L., & Sun, B. (2021). Comprehensive Pan-Cancer Analysis on CBX3 as a Prognostic and Immunological Biomarker.

He, Z., Chen, J., Chen, X., Wang, H., Tang, L., & Han, C. (2021). microRNA‐377 acts as a suppressor in esophageal squamous cell carcinoma through CBX3‐dependent P53/P21 pathway. Journal of cellular physiology, 236(1), 107-120.

Lin, H., Lian, J., Xia, L., Guan, G., & You, J. (2020). CBX3 promotes gastric cancer progression and affects factors related to immunotherapeutic responses. Cancer management and research, 12, 10113.

Bagheri, L., Thaler, R., Badreldin, A., Paradise, C. R., Khani, F., Deyle, D., ... & van Wijnen, A. (2020). Epigenetic Control of Osteoblast Differentiation by Chromobox 3 (Cbx3) Protein 3. The FASEB Journal, 34(S1), 1-1.

Lin, H., Zhao, X., Xia, L., Lian, J., & You, J. (2020). Clinicopathological and Prognostic Significance of CBX3 Expression in Human Cancer: a Systematic Review and Meta-analysis. Disease markers, 2020.

Liu, J., Zhan, Y., Wang, J., Wang, J., Guo, J., & Kong, D. (2020). lncRNA-SNHG17 promotes colon adenocarcinoma progression and serves as a sponge for miR-375 to regulate CBX3 expression. American Journal of Translational Research, 12(9), 5283.

Ma, C., Nie, X. G., Wang, Y. L., Liu, X. H., Liang, X., Zhou, Q. L., & Wu, D. P. (2019). CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma. Molecular medicine reports, 19(5), 4205-4212.

Zhang, H., Chen, W., Fu, X., Su, X., & Yang, A. (2018). CBX3 promotes tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma. Gene, 654, 49-56.

Meng, L., Wang, F., Sun, S., Zheng, Y., Ding, Z., Sun, Y., ... & Xu, M. (2017). MicroRNA-30b targets CBX3 and regulates cell proliferation, apoptosis, and migration in esophageal squamous cell carcinoma via the JAK2/STAT3 signaling pathway. International journal of clinical and experimental pathology, 10(12), 11828.

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For research use only. Not intended for any clinical use.

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