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Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY)


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Published Data
CBMAB-H0555-FY-1
Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY) in IF
Detection of nucleoli with HP1γ. HeLa cells were stained with antibodies against CAS, HP1γ and nucleolin (NCL). Panels depict single staining or different overlays as indicated in the figure. Size bar is 20 µm. ...View More
Su, H., Kodiha, M., Lee, S., & Stochaj, U. (2013). Identification of novel markers that demarcate the nucleolus during severe stress and chemotherapeutic treatment. PLoS One, 8(11), e80237.
CBMAB-H0555-FY-2
Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY) in IF
Confocal microscopy images of 2BS cells expressing scramble, shSTAT3, or shIL-6 upon exposure to RasV12. Immunofluorescence was performed for HP1γ (green), and DAPI staining (blue) was used to visualize DNA. ...View More
Jin, B., Wang, Y., Wu, C. L., Liu, K. Y., Chen, H., & Mao, Z. B. (2014). PIM-1 modulates cellular senescence and links IL-6 signaling to heterochromatin formation. Aging cell, 13(5), 879-889.
CBMAB-H0555-FY-3
Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY) in WB
Western blot of proteins pulled down with GST-tagged HP1γ wild-type, S93E or S93A mutant proteins from nuclear extracts of ras-induced senescent cells. Protein pull-down was probed with antibodies against HP1γ (top row), histone H3K9me3 (middle row), or histone H3 (bottom row). ...View More
Jin, B., Wang, Y., Wu, C. L., Liu, K. Y., Chen, H., & Mao, Z. B. (2014). PIM-1 modulates cellular senescence and links IL-6 signaling to heterochromatin formation. Aging cell, 13(5), 879-889.
CBMAB-H0555-FY-4
Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY) in IP
Lysates of 293 T cells expressing indicated GFP-PRR14 (Citation1-212) constructs were immunoprecipitated (IP) with anti-GFP antibody and analyzed by Western blotting using antibodies against the indicated HP1 isoform. IgG serves as an isotype control for nonspecific antibody interactions. ...View More
Kiseleva, A. A., Cheng, Y. C., Smith, C. L., Katz, R. A., & Poleshko, A. (2023). PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina. Nucleus, 14(1), 2165602.
CBMAB-H0555-FY-5
Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY) in IF
Representative single z-plane confocal images of parental and HP1γKD MDA-MB-231 cells. ...View More
Zhang, L., Geng, X., Wang, F., Tang, J., Ichida, Y., Sharma, A., ... & Zhang, Y. (2022). 53BP1 regulates heterochromatin through liquid phase separation. Nature communications, 13(1), 360.
CBMAB-H0555-FY-6
Mouse Anti-CBX3 Recombinant Antibody (CBFYH-0050) (CBMAB-H0555-FY) in WB
Western blot result of H3K9me3 and HP1 expression in negative control and ASF1a knockdown HepG2 and LNCap cells. ...View More
Wu, Y., Li, X., Yu, J., Björkholm, M., & Xu, D. (2019). ASF1a inhibition induces p53-dependent growth arrest and senescence of cancer cells. Cell death & disease, 10(2), 76.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYH-0050
Antibody Isotype
IgG2b, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Amino acids 6-25 at the N-terminus of HP1γ of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 29-86

Target

Full Name
Chromobox 3
Introduction
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Entrez Gene ID
Human11335
Mouse12417
Rat297093
UniProt ID
HumanQ13185
MouseP23198
RatQ5RJK5
Alternative Names
Chromobox 3
Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
Biological Process
Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin remodeling Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of transcription, DNA-templated Source: UniProtKB
Rhythmic process Source: UniProtKB-KW
Cellular Location
Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).
PTM
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
More Infomation

Huang, Y., Lin, Y., Song, X., & Wu, D. (2021). LINC00857 contributes to proliferation and lymphomagenesis by regulating miR-370-3p/CBX3 axis in diffuse large B-cell lymphoma. Carcinogenesis, 42(5), 733-741.

Niu, H., Chen, P., Fan, L., & Sun, B. (2021). Comprehensive Pan-Cancer Analysis on CBX3 as a Prognostic and Immunological Biomarker.

He, Z., Chen, J., Chen, X., Wang, H., Tang, L., & Han, C. (2021). microRNA‐377 acts as a suppressor in esophageal squamous cell carcinoma through CBX3‐dependent P53/P21 pathway. Journal of cellular physiology, 236(1), 107-120.

Lin, H., Lian, J., Xia, L., Guan, G., & You, J. (2020). CBX3 promotes gastric cancer progression and affects factors related to immunotherapeutic responses. Cancer management and research, 12, 10113.

Bagheri, L., Thaler, R., Badreldin, A., Paradise, C. R., Khani, F., Deyle, D., ... & van Wijnen, A. (2020). Epigenetic Control of Osteoblast Differentiation by Chromobox 3 (Cbx3) Protein 3. The FASEB Journal, 34(S1), 1-1.

Lin, H., Zhao, X., Xia, L., Lian, J., & You, J. (2020). Clinicopathological and Prognostic Significance of CBX3 Expression in Human Cancer: a Systematic Review and Meta-analysis. Disease markers, 2020.

Liu, J., Zhan, Y., Wang, J., Wang, J., Guo, J., & Kong, D. (2020). lncRNA-SNHG17 promotes colon adenocarcinoma progression and serves as a sponge for miR-375 to regulate CBX3 expression. American Journal of Translational Research, 12(9), 5283.

Ma, C., Nie, X. G., Wang, Y. L., Liu, X. H., Liang, X., Zhou, Q. L., & Wu, D. P. (2019). CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma. Molecular medicine reports, 19(5), 4205-4212.

Zhang, H., Chen, W., Fu, X., Su, X., & Yang, A. (2018). CBX3 promotes tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma. Gene, 654, 49-56.

Meng, L., Wang, F., Sun, S., Zheng, Y., Ding, Z., Sun, Y., ... & Xu, M. (2017). MicroRNA-30b targets CBX3 and regulates cell proliferation, apoptosis, and migration in esophageal squamous cell carcinoma via the JAK2/STAT3 signaling pathway. International journal of clinical and experimental pathology, 10(12), 11828.

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For research use only. Not intended for any clinical use.

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