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Mouse Anti-CAST Recombinant Antibody (CBLNC-136) (CBMAB-1318-CN)


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Published Data
CBMAB-1318-CN-1
Mouse Anti-CAST Recombinant Antibody (CBLNC-136) (CBMAB-1318-CN) in WB
Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and calpastatin (CAST) expression. GAPDH was used as loading control. ...View More
Stillger, M. N., Chen, C. Y., Lai, Z. W., Li, M., Schäfer, A., Pagenstecher, A., ... & Schilling, O. (2023). Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis. Cancer cell international, 23(1), 49.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLNC-136
Antibody Isotype
IgG1, κ
Application
ELISA, IHC-P, IF, IP, WB

Basic Information

Immunogen
Amino acids 409-708 of Calpastatin of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calpastatin
Introduction
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor, which consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4). Calpastatin is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation.
Entrez Gene ID
Human831
Cattle281039
Rat25403
Dog479148
Pig397135
Rabbit100009098
UniProt ID
HumanP20810
CattleA0A4W2IP19
RatP27321
DogE2QXA6
PigP12675
RabbitP08855
Alternative Names
BS-17; PLACK
Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Biological Process
Inhibition of cysteine-type endopeptidase activity Source: CAFA
Negative regulation of type B pancreatic cell apoptotic process Source: BHF-UCL
Presynaptic active zone organization Source: ARUK-UCL
Cellular Location
Cytosol; Endoplasmic reticulum; Cytoplasm; Membrane
Involvement in disease
Peeling skin with leukonychia, acral punctate keratoses, cheilitis, and knuckle pads (PLACK): An autosomal recessive disease characterized by generalized, continuous shedding of the outer layers of the epidermis, leukonychia, acral punctate keratosis, cheilitis, knuckle pads with multiple hyperkeratotic micropapules involving the interphalangeal joints, and palmoplantar keratoderma.
PTM
The N-terminus is blocked.
More Infomation

Flores-Toro, J., Chun, S. K., Shin, J. K., Campbell, J., Lichtenberger, M., Chapman, W., ... & Kim, J. S. (2021). Critical Roles of Calpastatin in Ischemia/Reperfusion Injury in Aged Livers. Cells, 10(8), 1863.

Hu, D., Sun, X., Magpusao, A., Fedorov, Y., Thompson, M., Wang, B., ... & Qi, X. (2021). Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington’s disease. Nature communications, 12(1), 1-19.

Motter, M. M., Corva, P. M., & Soria, L. A. (2021). Expression of calpastatin isoforms in three skeletal muscles of Angus steers and their association with fiber type composition and proteolytic potential. Meat Science, 171, 108267.

Bozhilova-Sakova, M., Dimitrova, I., Tzonev, T. I., & Petrov, N. (2020). Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science, 26(2), 475-479.

Burchett, R., Brun, M., Monckton, E. A., Poon, H. Y., & Godbout, R. (2019). Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells. Journal of Biological Chemistry, 294(4), 1173-1188.

Wang, S., Huang, Y., Yan, Y., Zhou, H., Wang, M., Liao, L., ... & Xiong, K. (2019). Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons. Annals of Anatomy-Anatomischer Anzeiger, 221, 57-67.

Jawasreh, K. I., & Ismail, Z. B. (2019). Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs. Journal of advanced veterinary and animal research, 6(2), 193.

Zhang, J., Wu, D., Li, Y., Fan, Y., Chen, H., & Xu, J. (2018). Evaluating the association between calpastatin (CAST) gene and keratoconus in the Han Chinese population. Gene, 653, 10-13.

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For research use only. Not intended for any clinical use.

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