Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CAMKK2 Recombinant Antibody (4C7) (CBMAB-0421-CN)


See all CAMKK2 antibodies
Online Inquiry
Published Data
CBMAB-0421-CN-1
Mouse Anti-CAMKK2 Recombinant Antibody (4C7) (CBMAB-0421-CN) in Immunoblot
Immunoblots were probed with antibodies directed against pLKB1, pACC, pACC, pVASP, total AMPK, ACC, VASP, CaMKKβ, Rac, or actin, as indicated. Results from a representative experiment. ...View More
Kou, R., Sartoretto, J., & Michel, T. (2009). Regulation of Rac1 by simvastatin in endothelial cells: differential roles of AMP-activated protein kinase and calmodulin-dependent kinase kinase-β. Journal of Biological Chemistry, 284(22), 14734-14743.
CBMAB-0421-CN-2
Mouse Anti-CAMKK2 Recombinant Antibody (4C7) (CBMAB-0421-CN) in Immunoblot
Cultured endothelial cells were transfected with control or CaMKKβ siRNA; 24 h later simvastatin (10 μm) was added, and cells were harvested 24 h later. Cell lysates were resolved by SDS-PAGE and analyzes in immunoblots probed with antibodies directed against phospho-LKB1, phospho-AMPK phospho-VASP, AMPK, VASP, ACC, Rac, actin, and CaMKKβ, as shown. A, a representative experiment; B, pooled data from four experiments showing quantitative analyses of phosphoprotein abundance in the presence and absence of simvastatin, for control and CaMKKβ-transfected cells. Basal AMPK phosphorylation in vehicle-treated cells was defined as 1.0; *, p < 0.05 (ANOVA, n = 4). ...View More
Kou, R., Sartoretto, J., & Michel, T. (2009). Regulation of Rac1 by simvastatin in endothelial cells: differential roles of AMP-activated protein kinase and calmodulin-dependent kinase kinase-β. Journal of Biological Chemistry, 284(22), 14734-14743.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
4C7
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
CAMKK2 (AAH26060, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcium/Calmodulin Dependent Protein Kinase Kinase 2
Introduction
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. PCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. This phosphorylation is stimulated in response to Ca2+ signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
Entrez Gene ID
10645
UniProt ID
Q96RR4
Alternative Names
Calcium/Calmodulin-Dependent Protein Kinase Kinase 2, Beta; CaM-Kinase Kinase Beta; CaM-Kinase Kinase 2; CaM-KK Beta; CaM-KK 2; CAMKKB; Calcium/Calmodulin-Dependent Protein Kinase Kinase Beta; Calcium/Calmodulin-Dependent Protein Kinase Beta; CAMKK Beta Protein; EC 2.7.11.17; CaMKK Beta; EC 2.7.11; KIAA0787; CaMKK 2; CAMKK
Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca2+ signals (By similarity).
Seems to be involved in hippocampal activation of CREB1 (By similarity).
May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
Biological Process
Calcium-mediated signaling Source: UniProtKB
CAMKK-AMPK signaling cascade Source: ParkinsonsUK-UCL
Cellular response to reactive oxygen species Source: ParkinsonsUK-UCL
Chemical synaptic transmission Source: Reactome
MAPK cascade Source: UniProtKB
Positive regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
Positive regulation of protein phosphorylation Source: ParkinsonsUK-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of protein kinase activity Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Neuron projection. Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction.
PTM
Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation.
More Infomation

Wang, S., Yi, X., Wu, Z., Guo, S., Dai, W., Wang, H., ... & Li, C. (2021). CAMKK2 Defines Ferroptosis Sensitivity of Melanoma Cells by Regulating AMPK‒NRF2 Pathway. Journal of Investigative Dermatology.

Zhang, Y., Recouvreux, M. V., Jung, M., Galenkamp, K. M., Li, Y., Zagnitko, O., ... & Commisso, C. (2021). Macropinocytosis in Cancer-Associated Fibroblasts is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness. Cancer Discovery.

Langendorf, C. G., O'Brien, M. T., Ngoei, K. R., McAloon, L. M., Dhagat, U., Hoque, A., ... & Scott, J. W. (2020). CaMKK2 is inactivated by cAMP-PKA signaling and 14-3-3 adaptor proteins. Journal of Biological Chemistry, 295(48), 16239-16250.

Spengler, K., Zibrova, D., Woods, A., Langendorf, C. G., Scott, J. W., Carling, D., & Heller, R. (2020). Protein kinase A negatively regulates VEGF-induced AMPK activation by phosphorylating CaMKK2 at serine 495. Biochemical Journal, 477(17), 3453-3469.

Lentini Santo, D., Petrvalska, O., Obsilova, V., Ottmann, C., & Obsil, T. (2020). Stabilization of Protein–Protein Interactions between CaMKK2 and 14–3–3 by Fusicoccins. ACS chemical biology, 15(11), 3060-3071.

O’Byrne, S. N., Scott, J. W., Pilotte, J. R., Santiago, A. D. S., Langendorf, C. G., Oakhill, J. S., ... & Drewry, D. H. (2020). In depth analysis of kinase cross screening data to identify CAMKK2 inhibitory scaffolds. Molecules, 25(2), 325.

Racioppi, L., Nelson, E. R., Huang, W., Mukherjee, D., Lawrence, S. A., Lento, W., ... & McDonnell, D. P. (2019). CaMKK2 in myeloid cells is a key regulator of the immune-suppressive microenvironment in breast cancer. Nature communications, 10(1), 1-16.

Tojkander, S., Ciuba, K., & Lappalainen, P. (2018). CaMKK2 regulates mechanosensitive assembly of contractile actin stress fibers. Cell reports, 24(1), 11-19.

Penfold, L., Woods, A., Muckett, P., Nikitin, A. Y., Kent, T. R., Zhang, S., ... & Carling, D. (2018). CAMKK2 promotes prostate cancer independently of AMPK via increased lipogenesis. Cancer research, 78(24), 6747-6761.

Jin, L., Chun, J., Pan, C., Kumar, A., Zhang, G., Ha, Y., ... & Kang, S. (2018). The PLAG1-GDH1 axis promotes anoikis resistance and tumor metastasis through CamKK2-AMPK signaling in LKB1-deficient lung cancer. Molecular cell, 69(1), 87-99.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CAMKK2 Recombinant Antibody (4C7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry