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Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY)


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Published Data
CBMAB-C0834-FY-1
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in WB
Western blot of candidate CaMKK2 KO MEFs generated via CRISPR/Cas9. GuideC was selected for further use. ...View More
Schmitt, D. L., Curtis, S. D., Lyons, A. C., Zhang, J. F., Chen, M., He, C. Y., ... & Zhang, J. (2022). Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter. Nature communications, 13(1), 3856.
CBMAB-C0834-FY-2
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in Immunoblot
Immunoblots showing ATP5A (A), GAPDH (B), and SDHB (A, B) protein levels in the SDHB knockdown (A) and SDHB overexpressed ER/mitochondrial fractions derived from CAMKK2−/− HEK293 and HepG2 cells, respectively. ...View More
Sabbir, M. G., Taylor, C. G., & Zahradka, P. (2021). CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner. Cell Communication and Signaling, 19, 1-29.
CBMAB-C0834-FY-3
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in Immunoblot
Immunoblot showing relative expression/abundance of RFP-TFRC (a), TF(b-c), CAMKK2 (d), CAMK4(e) in the wild-type, CAMKK2−/−, CAMK4−/−, and DKO HEK293 cells treated with 25 μg/ml partially iron-saturated human TF. ...View More
Sabbir, M. G. (2020). CAMKK2-CAMK4 signaling regulates transferrin trafficking, turnover, and iron homeostasis. Cell Communication and Signaling, 18, 1-21.
CBMAB-C0834-FY-4
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in WB
Inhibition of CAMKK2 by STO-609 inhibits phosphorylation of AMPK in SUM159PT, a CL-TNBC cell line. ...View More
Casciano, J. C., Perry, C., Cohen-Nowak, A. J., Miller, K. D., Vande Voorde, J., Zhang, Q., ... & Schug, Z. T. (2020). MYC regulates fatty acid metabolism through a multigenic program in claudin-low triple negative breast cancer. British journal of cancer, 122(6), 868-884.
CBMAB-C0834-FY-5
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in WB
Biliverdin activates mTORC2 and AMPK signaling in leukocytes. Healthy donor's blood was untreated or treated in vitro. Then, leukocytes were isolated, lysed, normalized for protein content and analyzed by western blotting. ...View More
Zhang, Z., Amorosa, L. F., Petrova, A., Coyle, S., Macor, M., Nair, M., ... & Haimovich, B. (2019). TLR4 counteracts BVRA signaling in human leukocytes via differential regulation of AMPK, mTORC1 and mTORC2. Scientific reports, 9(1), 7020.
CBMAB-C0834-FY-6
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in Immunoblot
Immunoblotting of control (shLuc), CaMKKβ knockdown (#1) restored with vector and CaMKKβ MDA-MB-231 cells serum starved and treated with EGF for 10 min. In each restoration experiment, 5 μg of the plasmid was transfected into indicated cell lines. ...View More
Han, F., Li, C. F., Cai, Z., Zhang, X., Jin, G., Zhang, W. N., ... & Lin, H. K. (2018). The critical role of AMPK in driving Akt activation under stress, tumorigenesis and drug resistance. Nature communications, 9(1), 4728.
CBMAB-C0834-FY-7
Mouse Anti-CAMKK2 Recombinant Antibody (CBFYC-0779) (CBMAB-C0834-FY) in IF
Live confocal fluorescent images showing localization of FITC-TF and RFP-TFR in the wild-type and CaMKK2 KO cells. The red arrow indicates the membrane localization of TFR. ...View More
Sabbir, M. G. (2018). Loss of Ca2+/calmodulin dependent protein kinase kinase 2 leads to aberrant transferrin phosphorylation and trafficking: a potential biomarker for Alzheimer's disease. Frontiers in molecular biosciences, 5, 99.

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBFYC-0779
Antibody Isotype
IgG1, κ
Application
ELISA, IP, WB

Basic Information

Immunogen
Recombinant CaMKKβ of human origin.
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcium/Calmodulin Dependent Protein Kinase Kinase 2
Introduction
This gene encodes a class I protein methyltransferase that acts in the formation of trimethyllysine in calmodulin. The protein contains a AdoMet-binding motif and may play a role in calcium-dependent signaling.
Entrez Gene ID
Human10645
Mouse207565
UniProt ID
HumanQ96RR4
MouseQ8C078
Alternative Names
Calcium/Calmodulin Dependent Protein Kinase Kinase 2; Calcium/Calmodulin-Dependent Protein Kinase Kinase 2, Beta; Calcium/Calmodulin-Dependent Protein Kinase Kinase Beta; CaM-Kinase Kinase Beta; CaM-Kinase Kinase 2; CaM-KK Beta; CaM-KK 2; CAMKKB; Calcium/Calmodulin-Dependent Protein Kinase Kinase 2
Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca2+ signals (By similarity).
Seems to be involved in hippocampal activation of CREB1 (By similarity).
May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
Biological Process
Calcium-mediated signaling Source: UniProtKB
CAMKK-AMPK signaling cascade Source: ParkinsonsUK-UCL
Cellular response to reactive oxygen species Source: ParkinsonsUK-UCL
Chemical synaptic transmission Source: Reactome
MAPK cascade Source: UniProtKB
Positive regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
Positive regulation of protein phosphorylation Source: ParkinsonsUK-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of protein kinase activity Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Neuron projection. Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction.
PTM
Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation.
More Infomation

Wang, S., Yi, X., Wu, Z., Guo, S., Dai, W., Wang, H., ... & Li, C. (2021). CAMKK2 Defines Ferroptosis Sensitivity of Melanoma Cells by Regulating AMPK‒NRF2 Pathway. Journal of Investigative Dermatology.

Zhang, Y., Recouvreux, M. V., Jung, M., Galenkamp, K. M., Li, Y., Zagnitko, O., ... & Commisso, C. (2021). Macropinocytosis in Cancer-Associated Fibroblasts is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness. Cancer Discovery.

Langendorf, C. G., O'Brien, M. T., Ngoei, K. R., McAloon, L. M., Dhagat, U., Hoque, A., ... & Scott, J. W. (2020). CaMKK2 is inactivated by cAMP-PKA signaling and 14-3-3 adaptor proteins. Journal of Biological Chemistry, 295(48), 16239-16250.

Spengler, K., Zibrova, D., Woods, A., Langendorf, C. G., Scott, J. W., Carling, D., & Heller, R. (2020). Protein kinase A negatively regulates VEGF-induced AMPK activation by phosphorylating CaMKK2 at serine 495. Biochemical Journal, 477(17), 3453-3469.

Lentini Santo, D., Petrvalska, O., Obsilova, V., Ottmann, C., & Obsil, T. (2020). Stabilization of Protein–Protein Interactions between CaMKK2 and 14–3–3 by Fusicoccins. ACS chemical biology, 15(11), 3060-3071.

O’Byrne, S. N., Scott, J. W., Pilotte, J. R., Santiago, A. D. S., Langendorf, C. G., Oakhill, J. S., ... & Drewry, D. H. (2020). In depth analysis of kinase cross screening data to identify CAMKK2 inhibitory scaffolds. Molecules, 25(2), 325.

Racioppi, L., Nelson, E. R., Huang, W., Mukherjee, D., Lawrence, S. A., Lento, W., ... & McDonnell, D. P. (2019). CaMKK2 in myeloid cells is a key regulator of the immune-suppressive microenvironment in breast cancer. Nature communications, 10(1), 1-16.

Tojkander, S., Ciuba, K., & Lappalainen, P. (2018). CaMKK2 regulates mechanosensitive assembly of contractile actin stress fibers. Cell reports, 24(1), 11-19.

Penfold, L., Woods, A., Muckett, P., Nikitin, A. Y., Kent, T. R., Zhang, S., ... & Carling, D. (2018). CAMKK2 promotes prostate cancer independently of AMPK via increased lipogenesis. Cancer research, 78(24), 6747-6761.

Jin, L., Chun, J., Pan, C., Kumar, A., Zhang, G., Ha, Y., ... & Kang, S. (2018). The PLAG1-GDH1 axis promotes anoikis resistance and tumor metastasis through CamKK2-AMPK signaling in LKB1-deficient lung cancer. Molecular cell, 69(1), 87-99.

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For research use only. Not intended for any clinical use.

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