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Mouse Anti-CACYBP Recombinant Antibody (CBXS-1036) (CBMAB-S3929-CQ)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXS-1036
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 1-228 representing full length CacyBP of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcyclin Binding Protein
Introduction
The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
27101
UniProt ID
Q9HB71
Alternative Names
Calcyclin Binding Protein; S100A6-Binding Protein; S100A6BP; HCacyBP; SIP; Growth-Inhibiting Gene 5 Protein; Siah-Interacting Protein (SIP);
Function
May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Biological Process
Aging Source: Ensembl
Cardiac muscle cell differentiation Source: Ensembl
Cellular response to calcium ion Source: Ensembl
Cellular response to leukemia inhibitory factor Source: Ensembl
Heart development Source: GO_Central
Negative regulation of cell death Source: Ensembl
Positive regulation of DNA replication Source: Ensembl
Response to growth hormone Source: Ensembl
Cellular Location
Nucleus; Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
PTM
Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
More Infomation

Zheng, H., & Chen, C. (2021). Downregulation of CacyBP by CRISPR/dCas9-KRAB Prevents Bladder Cancer Progression. Frontiers in Molecular Biosciences, 8, 557.

Xu, Y. J., Hu, Y. M., Qin, C., Wang, F., Cao, W., Yu, Y. W., ... & He, J. (2021). CacyBP promotes the proliferation and invasion of non-small cell lung cancer. Zhonghua Zhong liu za zhi [Chinese Journal of Oncology], 43(9), 924-931.

Zhao, M., Zhang, R. Z., Qi, D. W., Chen, H. Y., & Zhang, G. C. (2020). CacyBP/SIP promotes tumor progression by regulating apoptosis and arresting the cell cycle in osteosarcoma. Experimental and Therapeutic Medicine, 20(2), 1397-1404.

Lian, Y. F., Huang, Y. L., Zhang, Y. J., Chen, D. M., Wang, J. L., Wei, H., ... & Huang, Y. H. (2019). CACYBP enhances cytoplasmic retention of P27Kip1 to promote hepatocellular carcinoma progression in the absence of RNF41 mediated degradation. Theranostics, 9(26), 8392.

Kasacka, I., Piotrowska, Ż., Weresa, J., & Filipek, A. (2018). Comparative evaluation of CacyBP/SIP protein, β-catenin, and immunoproteasome subunit LMP7 in the heart of rats with hypertension of different etiology. Experimental Biology and Medicine, 243(15-16), 1199-1206.

Feng, S., Zhou, Q., Yang, B., Li, Q., Liu, A., Zhao, Y., ... & Zhai, H. (2018). The effect of S100A6 on nuclear translocation of CacyBP/SIP in colon cancer cells. Plos one, 13(3), e0192208.

Yan, S., Li, A., & Liu, Y. (2018). CacyBP/SIP inhibits the migration and invasion behaviors of glioblastoma cells through activating Siah1 mediated ubiquitination and degradation of cytoplasmic p27. Cell biology international, 42(2), 216-226.

Topolska-Woś, A. M., Rosińska, S., & Filipek, A. (2017). MAP kinase p38 is a novel target of CacyBP/SIP phosphatase. Amino Acids, 49(6), 1069-1076.

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For research use only. Not intended for any clinical use.

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