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Rat Anti-C5AR2 Recombinant Antibody (CBLG1-352) (CBMAB-G1312-LY)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBLG1-352
Antibody Isotype
IgG2b
Application
FC

Basic Information

Immunogen
Chinese hamster ovary cell line transfected with mouse C5L2 Met1-Val344.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC0.25 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
complement component 5a receptor 2
Introduction
This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
319430
UniProt ID
Q8BW93
Alternative Names
C5L2; E030029A11Rik; Gpr77
Function
Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways.
Biological Process
Chemotaxis Source: InterPro
Complement receptor mediated signaling pathway Source: GO_Central
G protein-coupled receptor signaling pathway Source: Reactome
Inflammatory response Source: GO_Central
Negative regulation of interleukin-6 production Source: UniProtKB
Negative regulation of neutrophil chemotaxis Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: UniProtKB
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of epithelial cell proliferation Source: InterPro
Positive regulation of ERK1 and ERK2 cascade Source: InterPro
Regulation of complement activation Source: Reactome
Regulation of interleukin-8 production Source: UniProtKB
Cellular Location
Cell membrane
Topology
Extracellular: 1-38 aa
Helical: 39-61 aa
Cytoplasmic: 62-72 aa
Helical: 73-95 aa
Extracellular: 96-114 aa
Helical: 115-137 aa
Cytoplasmic: 138-149 aa
Helical: 150-172 aa
Extracellular: 173-202 aa
Helical: 203-225 aa
Cytoplasmic: 226-237 aa
Helical: 238-260 aa
Extracellular: 261-274 aa
Helical: 275-294 aa
Cytoplasmic: 295-337 aa
More Infomation

Wu, M. C., Lee, J. D., Ruitenberg, M. J., & Woodruff, T. M. (2020). Absence of the C5a Receptor C5aR2 Worsens Ischemic Tissue Injury by Increasing C5aR1-Mediated Neutrophil Infiltration. The Journal of Immunology, 205(10), 2834-2839.

Miyabe, Y., Miyabe, C., Mani, V., Mempel, T. R., & Luster, A. D. (2019). Atypical complement receptor C5aR2 transports C5a to initiate neutrophil adhesion and inflammation. Science immunology, 4(35).

Li, X. X., Lee, J. D., Kemper, C., & Woodruff, T. M. (2019). The complement receptor C5aR2: a powerful modulator of innate and adaptive immunity. The Journal of Immunology, 202(12), 3339-3348.

Nabizadeh, J. A., Manthey, H. D., Panagides, N., Steyn, F. J., Lee, J. D., Li, X. X., ... & Rolfe, B. E. (2019). C5a receptors C5aR1 and C5aR2 mediate opposing pathologies in a mouse model of melanoma. The FASEB Journal, 33(10), 11060-11071.

Verghese, D. A., Demir, M., Chun, N., Fribourg, M., Cravedi, P., Llaudo, I., ... & Heeger, P. S. (2018). T cell expression of C5a receptor 2 augments murine regulatory T cell (TREG) generation and TREG-dependent cardiac allograft survival. The Journal of Immunology, 200(6), 2186-2198.

Orrem, H. L., Nilsson, P. H., Pischke, S. E., Kleveland, O., Yndestad, A., Ekholt, K., ... & Mollnes, T. E. (2018). IL-6 receptor inhibition by tocilizumab attenuated expression of C5a receptor 1 and 2 in non-ST-elevation myocardial infarction. Frontiers in immunology, 9, 2035.

Zhang, T., Garstka, M. A., & Li, K. (2017). The controversial C5a receptor C5aR2: its role in health and disease. Journal of immunology research, 2017.

Kovtun, A., Bergdolt, S., Hägele, Y., Matthes, R., Lambris, J. D., Huber-Lang, M., & Ignatius, A. (2017). Complement receptors C5aR1 and C5aR2 act differentially during the early immune response after bone fracture but are similarly involved in bone repair. Scientific reports, 7(1), 1-12.

Poppelaars, F., van Werkhoven, M. B., Kotimaa, J., Veldhuis, Z. J., Ausema, A., Broeren, S. G., ... & Seelen, M. A. (2017). Critical role for complement receptor C5aR2 in the pathogenesis of renal ischemia‐reperfusion injury. The FASEB Journal, 31(7), 3193-3204.

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For research use only. Not intended for any clinical use.

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