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Mouse Anti-BRD3 Recombinant Antibody (CBYY-2148) (CBMAB-2497-YY)


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Published Data
CBMAB-2497-YY-1
CBMAB-2497-YY in WB
Statistical significance was indicated by an asterisk to highlight when the BRD4 expression was compared to BRD2 or BRD3 gene expression. The levels of BRD2, BRD3 or BRD4 protein expression were analyzed by western blots in OE33 cell line under downregulation of BRD2, BRD3, or BRD4 by siRNA (N = 3). ...View More
Alvarez-Trotta, A., Wang, Z., Shersher, E., Li, B., Long, J., Lohse, I., ... & Capobianco, A. J. (2020). The bromodomain inhibitor IBET-151 attenuates vismodegib-resistant esophageal adenocarcinoma growth through reduction of GLI signaling. Oncotarget, 11(33), 3174.
CBMAB-2497-YY-2
CBMAB-2497-YY in Immunoblot
IB analysis of WCL and chromatin derived from BRD4WT and BRD43RK knock-in cells treated with 10 μM Eto for 2 hours before harvesting. ...View More
Liu, L., Lin, B., Yin, S., Ball, L. E., Delaney, J. R., Long, D. T., & Gan, W. (2022). Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair. Science Advances, 8(49), eadd8928.
CBMAB-2497-YY-3
CBMAB-2497-YY in WB
The expression changes of G6PD, PIK3CA, and BRD4 genes in MDA-MB-231 and MCF-7 after being treated with erastin for 48 h were detected by western blot. ...View More
Zhu, L., Tian, Q., Jiang, S., Gao, H., Yu, S., Zhou, Y., ... & Wang, B. (2021). A novel ferroptosis-related gene signature for overall survival prediction in patients with breast cancer. Frontiers in cell and developmental biology, 9, 670184.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-2148
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 175-192 within an internal region of BRD3 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Bromodomain Containing 3
Introduction
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
Entrez Gene ID
8019
UniProt ID
Q15059
Alternative Names
Bromodomain Containing 3; RING3-Like Protein; RING3L; Bromodomain-Containing Protein 3 Short Isoform; Bromodomain-Containing Protein 3; Bromodomain-Containing 3; RING3-Like; KIAA0043; ORFX;
Function
Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114).
Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
Biological Process
Chromatin organization Source: UniProtKB-KW
Regulation of transcription by RNA polymerase II Source: UniProtKB
Cellular Location
Nucleus. Detected on chromatin.
Involvement in disease
A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUTM1 which produces a BRD3-NUTM1 fusion protein.
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For research use only. Not intended for any clinical use.

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