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Rabbit Anti-BHMT Recombinant Antibody (CBYY-0554) (CBMAB-0557-YY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBYY-0554
Antibody Isotype
IgG
Application
WB, IHC, IP

Basic Information

Immunogen
A synthesized peptide derived from human BHMT.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
IHC1:50-1:200
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4, 50% Glycerol, 0.4-0.5mg/ml BSA
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Betaine--Homocysteine S-Methyltransferase
Introduction
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Entrez Gene ID
635
UniProt ID
Q93088
Alternative Names
Betaine--Homocysteine S-Methyltransferase; EC 2.1.1.5; Epididymis Secretory Sperm Binding Protein Li 61p; Betaine--Homocysteine S-Methyltransferase 1; Betaine Homocysteine Methyltransferase; HEL-S-61p; EC 2.1.1; BHMT1;
Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Biological Process
Amino-acid betaine catabolic process Source: UniProtKB-UniPathway
Amino-acid betaine metabolic process Source: BHF-UCL
Choline catabolic process Source: Reactome
L-methionine salvage Source: BHF-UCL
Protein methylation Source: UniProtKB
Regulation of homocysteine metabolic process Source: UniProtKB
Sulfur amino acid metabolic process Source: Reactome
Cellular Location
Cytoplasm
More Infomation

Sternbach, S., West, N., Singhal, N. K., Clements, R., Basu, S., Tripathi, A., ... & McDonough, J. (2021). The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PloS one, 16(5), e0250486.

Na, J. D., Choi, Y. J., Jun, D. S., & Kim, Y. C. (2019). Alleviation of paraquat-induced oxidative lung injury by betaine via regulation of sulfur-containing amino acid metabolism despite the lack of betaine-homocysteine methyltransferase (BHMT) in the lung. Food & function, 10(2), 1225-1234.

Maclean, K. N., Jiang, H., Phinney, W. N., Keating, A. K., Hurt, K. J., & Stabler, S. P. (2019). Taurine alleviates repression of betaine‐homocysteine S‐methyltransferase and significantly improves the efficacy of long‐term betaine treatment in a mouse model of cystathionine β‐synthase–deficient homocystinuria. The FASEB Journal, 33(5), 6339-6353.

Saande, C. J., Pritchard, S. K., Worrall, D. M., Snavely, S. E., Nass, C. A., Neuman, J. C., ... & Schalinske, K. L. (2019). Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats. The Journal of nutrition, 149(8), 1369-1376.

Partearroyo, T., Murillo‐Cuesta, S., Vallecillo, N., Bermúdez‐Muñoz, J. M., Rodríguez‐de LaRosa, L., Mandruzzato, G., ... & Varela‐Nieto, I. (2019). Betaine‐homocysteine S‐methyltransferase deficiency causes increased susceptibility to noise‐induced hearing loss associated with plasma hyperhomocysteinemia. The FASEB Journal, 33(5), 5942-5956.

Wu, L., Zhou, X., Li, T., He, J., Huang, L., Ouyang, Z., ... & He, Q. (2018). Improved Sp1 and betaine homocysteine-S-methyltransferase expression and homocysteine clearance are involved in the effects of zinc on oxidative stress in high-fat-diet-pretreated mice. Biological trace element research, 184(2), 436-441.

Garrido, F., Pacheco, M., Vargas-Martínez, R., Velasco-García, R., Jorge, I., Serrano, H., ... & Pajares, M. Á. (2018). Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase. PloS one, 13(6), e0199472.

Prieur, E. A., Pjetri, E., Zeisel, S. H., & Jadavji, N. M. (2017). Reduced brain volume and impaired memory in betaine homocysteine S-methyltransferase knockout mice. Applied Physiology, Nutrition, and Metabolism, 42(11), 1228-1231.

Pérez-Miguelsanz, J., Vallecillo, N., Garrido, F., Reytor, E., Pérez-Sala, D., & Pajares, M. A. (2017). Betaine homocysteine S-methyltransferase emerges as a new player of the nuclear methionine cycle. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1864(7), 1165-1182.

Mostashari-Mohases, M., Sadeghi, A. A., Ahmadi, J., & Esmaeilkhanian, S. (2017). Effect of Betaine Supplementation on Performance Parameters, Betaine-homocysteine S-methyltransferase Gene Expression in Broiler Chickens Consume Drinking Water with Different Total Dissolved Solids. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(4).

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For research use only. Not intended for any clinical use.

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